Peptide transport in rabbit intestinal brush-border membrane vesicles studied with a potential-sensitive dye.
Peptide transport in rabbit intestinal brush-border membrane vesicles studied with a potential-sensitive dye.
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DOI：
10.1016/0005-2736(85)90490-0
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发表时间：
1985-06
期刊：
影响因子：
--
通讯作者：
V. Ganapathy;G. Burckhardt;F. Leibach
中科院分区：
文献类型：
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作者：
V. Ganapathy;G. Burckhardt;F. Leibach
使用电位敏感荧光染料di-S-C3研究了纯化的兔肠刷状缘膜囊泡中的肽转运（5）。二肽的转运伴随着在存在和不存在Na+的情况下染料荧光的增加，表明产电的、Na+-非依赖性肽转运。含有d-氨基酸的二肽也增加荧光，表明这些肽对肽转运系统也具有显著的亲和力。β-丙氨酰甘氨酰甘氨酸和脯氨酰甘氨酰甘氨酸与二肽非常相似，即使在不存在Na+的情况下也会增加荧光，这证明了三肽的Na+非依赖性产电转运。然而，三肽的半最大荧光变化所需的浓度高于二肽，表明载体的亲和力不同。此外，本研究还为小肠刷状缘膜上存在多个小肽转运载体系统提供了证据。
Peptide transport in purified rabbit intestinal brush-border membrane vesicles has been studied using a potential-sensitive fluorescent dye, di-S-C3(5). Transport of dipeptides is accompanied by an increase in the fluorescence of the dye in the presence and absence of Na+, indicating electrogenic, Na+-independent peptide transport. Dipeptides containingd-amino acids also increase the fluorescence, showing that these peptides too possess significant affinity for the peptide transport system. β-Alanylglycylglycine and prolylglycylglycine, very much like the dipeptides, increase the fluorescence even in the absence of Na+which demonstrates the Na+-independent, electrogenic transport of tripeptides. However, concentrations needed for half-maximal fluorescence changes are higher for tripeptides than for dipeptides suggesting different affinities for the carriers. The studies, in addition, provide evidence for the existence of more than one carrier system for translocation of small peptides in rabbit intestinal brush-border membrane.