Mimicking the host and its microenvironment in vitro for studying mucosal infections by Pseudomonas aeruginosa.
Mimicking the host and its microenvironment in vitro for studying mucosal infections by Pseudomonas aeruginosa.
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DOI：
10.1111/2049-632x.12180
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发表时间：
2014-06
影响因子：
3.3
通讯作者：
Nickerson CA
中科院分区：
文献类型：
--
作者：
Crabbé A;Ledesma MA;Nickerson CA
为什么健康的人免受铜绿假单胞菌感染，而囊性纤维化或上皮受损的人特别容易受到这种机会性病原体的影响？为了解决这个问题，必须彻底了解宿主微环境和铜绿假单胞菌之间的动态相互作用。因此，使用代表健康和疾病中人类粘膜组织的关键方面的模型I系统允许以生理相关的方式重建体内宿主-病原体相互作用。在这篇综述中，我们讨论了粘膜组织的因素，如顶-底侧极性，连接复合物，细胞外基质蛋白，粘液，多细胞复杂性（包括土著微生物群），以及其他理化因素如何影响铜绿假单胞菌的发病机制，因此在体外模拟很重要。我们强调了在过去35年中用于研究铜绿假单胞菌感染性疾病过程的日益复杂的体外细胞和组织培养模型系统，主要集中在肺部模型，以及它们各自的优点和局限性。基于我们对参与铜绿假单胞菌发病机制的宿主微环境因素的不断扩展的知识，体外模型的持续改进将有助于推进对致病机制的基本理解，并增加研究结果从实验室到患者床边的转化潜力。
Why is a healthy person protected from Pseudomonas aeruginosa infections, while individuals with cystic fibrosis or damaged epithelium are particularly susceptible to this opportunistic pathogen? In order to address this question, it is essential to thoroughly understand the dynamic interplay between the host microenvironment and P. aeruginosa. Therefore, using modeI systems that represent key aspects of human mucosal tissues in health and disease allows recreating in vivo host-pathogen interactions in a physiologically relevant manner. In this review, we discuss how factors of mucosal tissues, such as apical-basolateral polarity, junctional complexes, extracellular matrix proteins, mucus, multicellular complexity (including indigenous microbiota), and other physicochemical factors affect P. aeruginosa pathogenesis and are thus important to mimic in vitro. We highlight in vitro cell and tissue culture model systems of increasing complexity that have been used over the past 35 years to study the infectious disease process of P. aeruginosa, mainly focusing on lung models, and their respective advantages and limitations. Continued improvements of in vitro models based on our expanding knowledge of host microenvironmental factors that participate in P. aeruginosa pathogenesis will help advance fundamental understanding of pathogenic mechanisms and increase the translational potential of research findings from bench to the patient’s bedside.
CF气道上皮细胞对PAO1依从性的夸张的细胞因子反应的关系。
影响因子：
5.8
作者：
Kube, DM;Fletcher, D;Davis, PB
通讯作者：
Davis, PB