Arabidopsis sirtuins and poly( ADP -ribose) polymerases regulate gene expression in the day but do not affect circadian rhythms
Arabidopsis sirtuins and poly( ADP -ribose) polymerases regulate gene expression in the day but do not affect circadian rhythms
复制标题
拟南芥 Sirtuins 和聚（ADP-核糖）聚合酶调节白天的基因表达，但不影响昼夜节律
DOI：
10.1111/pce.13996
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kim J
中科院分区：
文献类型：
--
作者：
Kim J
烟酰胺腺嘌呤二核苷酸（NAD）参与氧化还原稳态，并作为NAD酶的底物，包括将聚（ADP-核糖）聚合物添加到蛋白质和DNA的聚（ADP-核糖）聚合酶（PARP），以及使蛋白质脱乙酰的sirtuins。烟酰胺是NAD酶的副产物，可增加植物和动物的昼夜节律周期。在哺乳动物中，烟酰胺对昼夜节律周期的影响可能是由PARP和sirtuins介导的，因为它们直接与核心昼夜节律振荡基因结合。我们研究了PARP和sirtuins是否参与了拟南芥昼夜节律振荡器的调控。我们没有发现PARP和sirtuins调节拟南芥的昼夜节律振荡器或参与对烟酰胺的反应的证据。RNA测序分析表明，PARP仅调节少数基因的表达，包括FLOWERLOCUS C。然而，我们发现sirtuin 1表达减少对白天的基因表达有深远的影响，但对晚上的基因表达没有影响，并且在基因敲除中存在胚胎致死表型。我们的研究结果表明PARP和sirtuins与NAD对昼夜节律振荡器的调节无关，sirtuin 1与基因表达的日间调节有关。
Nicotinamide‐adenine dinucleotide (NAD) is involved in redox homeostasis and acts as a substrate for NADases, including poly(ADP‐ribose) polymerases (PARPs) that add poly(ADP‐ribose) polymers to proteins and DNA, and sirtuins that deacetylate proteins. Nicotinamide, a by‐product of NADases increases circadian period in both plants and animals. In mammals, the effect of nicotinamide on circadian period might be mediated by the PARPs and sirtuins because they directly bind to core circadian oscillator genes. We have investigated whether PARPs and sirtuins contribute to the regulation of the circadian oscillator in Arabidopsis. We found no evidence that PARPs and sirtuins regulate the circadian oscillator of Arabidopsis or are involved in the response to nicotinamide. RNA‐seq analysis indicated that PARPs regulate the expression of only a few genes, includingFLOWERING LOCUS C. However, we found profound effects of reducedsirtuin 1expression on gene expression during the day but not at night, and an embryo lethal phenotype in knockouts. Our results demonstrate that PARPs and sirtuins are not associated with NAD regulation of the circadian oscillator and thatsirtuin 1is associated with daytime regulation of gene expression.