Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells.
Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells.
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DOI：
10.3389/fgene.2017.00208
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发表时间：
2017
影响因子：
3.7
通讯作者：
Akimitsu N
中科院分区：
文献类型：
--
作者：
Tano K;Onoguchi-Mizutani R;Yeasmin F;Uchiumi F;Suzuki Y;Yada T;Akimitsu N
MALAT 1长非编码RNA与癌症进展密切相关。在这里，我们报告了MALAT 1在抑制p53（TP 53）肿瘤抑制基因启动子的功能。在A549人肺腺癌细胞中，MALAT 1敲低上调了p21和FAS（众所周知的p53靶点）。我们发现，这些上调介导的转录激活p53通过MALAT 1耗尽。此外，我们确定了一个最小的MALAT 1反应区的P1启动子的p53基因。流式细胞术分析显示MALAT 1-耗尽的细胞表现出G1细胞周期停滞。这些结果表明MALAT 1通过抑制p53启动子活性影响p53靶基因的表达，从而影响细胞周期进程。
The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.