Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions.
Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions.
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DOI：
10.7554/elife.56088
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发表时间：
2021-03-09
期刊：
影响因子：
7.7
通讯作者：
Hanke GT
中科院分区：
文献类型：
--
作者：
Kramer M;Rodriguez-Heredia M;Saccon F;Mosebach L;Twachtmann M;Krieger-Liszkay A;Duffy C;Knell RJ;Finazzi G;Hanke GT
在光合作用过程中，电子传递是碳同化所必需的，必须加以调节，以最大限度地减少自由基损伤。关于一个关键酶(铁氧还蛋白：NADP(H)氧化还原酶，或FNR)在这一过程中的作用，特别是它在叶绿体中的位置，长期以来一直存在争议。在这里，我们使用免疫金标记法来证明以前被指定为可溶性的FNR实际上是膜相关的。我们在模式植物拟南芥中将这一技术与遗传方法相结合，以表明该酶在不同膜区域之间的分布取决于它与特定系链蛋白的相互作用。我们进一步证明了FNR与不同蛋白质的相互作用与其他光合作用电子传递途径的活性之间的相关性。这支持了FNR定位在从暗到光的转换过程中调节光合作用电子流的作用。
During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.