Gene expression profiling reveals novel biomarkers in nonsmall cell lung cancer
Gene expression profiling reveals novel biomarkers in nonsmall cell lung cancer
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DOI：
10.1002/ijc.25704
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发表时间：
2011-07-15
影响因子：
6.4
通讯作者：
Esther Farez-Vidal, Ma
中科院分区：
文献类型：
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作者：
Sanchez-Palencia, Abel;Gomez-Morales, Mercedes;Esther Farez-Vidal, Ma
可靠的基因表达谱技术的发展对我们对肺癌生物学的理解产生了越来越大的影响。我们的研究旨在确定肺癌的表型异质性和遗传多样性之间的相关性。对46例非小细胞肺癌（NSCLC）肿瘤样本和45例配对非肿瘤样本进行微阵列分析，建立原发性腺癌和鳞状细胞癌的基因特征，确定不同疾病阶段差异表达的基因序列，并鉴定对肿瘤进展具有生物学意义的序列。微阵列分析后，在48个肿瘤样本和22个非肿瘤样本组成的70个独立样本中，通过qPCR和鲁棒Bonferroni测试验证92个选定基因的表达水平。基因序列的表达随肿瘤类型、分期和分化程度的不同而不同。KRT15和PKP1表达上调，可能是鉴别鳞状细胞癌样本的良好标志物。在IA期腺癌中观察到DSG3的高下调。
The development of reliable gene expression profiling technology is having an increasing impact on our understanding of lung cancer biology. Our study aimed to determine any correlation between the phenotypic heterogeneity and genetic diversity of lung cancer. Microarray analysis was performed on a set of 46 tumor samples and 45 paired nontumor samples of nonsmall cell lung cancer (NSCLC) samples to establish gene signatures in primary adenocarcinomas and squamous-cell carcinomas, determine differentially expressed gene sequences at different stages of the disease and identify sequences with biological significance for tumor progression. After the microarray analysis, the expression level of 92 selected genes was validated by qPCR and the robust Bonferroni test in an independent set of 70 samples composed of 48 tumor samples and 22 nontumor samples. Gene sequences were differentially expressed as a function of tumor type, stage and differentiation grade. High upregulation was observed for KRT15 and PKP1, which may be good markers to distinguish squamous-cell carcinoma samples. High downregulation was observed for DSG3 in stage IA adenocarcinomas.