Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation
Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation
复制标题
DOI：
10.1039/b804808h
复制
发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Yanik, Mehmet Fatih
中科院分区：
文献类型：
--
作者：
Zeng, Fei;Rohde, Christopher B.;Yanik, Mehmet Fatih
稳定、快速和可重复的小动物固定技术对于利用多细胞生物体中的细胞和亚细胞特征进行高通量体内遗传/药物筛选是必要的。我们展示了一种非侵入性和高通量的生理活性C的芯片上固定化方法。在不使用麻醉或冷却的情况下，该方法可以用于线虫，但即使对于最苛刻的目的，也具有相当的稳定性。我们使用双光子显微镜和飞秒激光显微手术观察和操纵固定动物的亚细胞特征。
Techniques for stable, rapid and repeatable small-animal immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multi-cellular organisms. We demonstrate a method for non-invasive and high-throughput on-chip immobilization of physiologically active C. elegans without the use of anesthesia or cooling, but with comparable stability even for the most demanding purposes. We show observation and manipulation of sub-cellular features in immobilized animals using two-photon microscopy and femtosecond-laser microsurgery.