DNA microarray analysis of functionally discrete human brain regions reveals divergent transcriptional profiles
DNA microarray analysis of functionally discrete human brain regions reveals divergent transcriptional profiles
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DOI：
10.1016/s0969-9961(03)00126-8
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发表时间：
2003-11-01
影响因子：
6.1
通讯作者：
Akil, H
中科院分区：
文献类型：
--
作者：
Evans, SJ;Choudary, PV;Akil, H
在离散的人类大脑区域的转录谱可能反映了结构和功能的专业化。利用DNA微阵列技术，本研究调查了高度分化的大脑区域（小脑皮层和大脑皮层）转录谱的差异，以及两个密切相关的大脑结构（前扣带皮层和背外侧前额叶皮层）之间的差异。本研究在三个独立实验室的复制，以解决假阳性和假阴性结果使用微阵列技术，也进行了讨论。我们发现在小脑和大脑皮层之间有超过1000个转录本的差异表达，而在两个新皮层区域之间有差异表达的转录本很少。我们进一步表征了被发现在被比较的大脑区域内特异性表达的转录本，并发现代表信号转导机制、神经发生、突触传递和转录因子的本体论类别最具代表性。(C) 2003 Elsevier Inc.版权所有。
Transcriptional profiles within discrete human brain regions are likely to reflect structural and functional specialization. Using DNA microarray technology, this study investigates differences in transcriptional profiles of highly divergent brain regions (the cerebellar cortex and the cerebral cortex) as well as differences between two closely related brain structures (the anterior cingulate cortex and the dorsolateral prefrontal cortex). Replication of this study across three independent laboratories, to address false-positive and false-negative results using microarray technology, is also discussed. We find greater than a thousand transcripts to be differentially expressed between cerebellum and cerebral cortex and very few transcripts to be differentially expressed between the two neocortical regions. We further characterized transcripts that were found to be specifically expressed within brain regions being compared and found that ontological classes representing signal transduction machinery, neurogenesis, synaptic transmission, and transcription factors were most highly represented. (C) 2003 Elsevier Inc. All rights reserved.