Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae:: Implications for the microbial "pan-genome"
Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae:: Implications for the microbial "pan-genome"
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DOI：
10.1073/pnas.0506758102
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发表时间：
2005-09-27
影响因子：
11.1
通讯作者：
Fraser, CM
中科院分区：
文献类型：
--
作者：
Tettelin, H;Masignani, V;Fraser, CM
高效且廉价的基因组测序方法的发展彻底改变了人类细菌病原体的研究，并改进了疫苗设计。不幸的是，单个基因组的序列并不能反映遗传变异性如何驱动细菌物种内的发病机制，也限制了候选疫苗或抗菌靶标的全基因组筛选。我们已经生成了代表无乳链球菌五种主要致病血清型的六种菌株的基因组序列，无乳链球菌是人类新生儿感染的主要原因。对这些基因组和数据库中的基因组的分析表明，S。无乳种可以通过由所有分离株共有的核心基因组组成的泛基因组（占任何单个基因组的约80%），加上由部分共有的和菌株特异性基因组成的杂合基因组来描述。数据的数学外推表明，S.无乳链球菌泛基因组是巨大的，即使在对数百个基因组进行测序后，也将继续鉴定出独特的基因。
The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single genome does not reflect how genetic variability drives pathogenesis within a bacterial species and also limits genomewide screens for vaccine candidates or for antimicrobial targets. We have generated the genomic sequence of six strains representing the five major disease-causing serotypes of Streptococcus agalactiae, the main cause of neonatal infection in humans. Analysis of these genomes and those available in databases showed that the S. agalactiae species can be described by a pan-genome consisting of a core genome shared by all isolates, accounting for approximate to 80% of any single genome, plus a dispensable genome consisting of partially shared and strain-specific genes. Mathematical extrapolation of the data suggests that the gene reservoir available for inclusion in the S. agalactiae pan-genome is vast and that unique genes will continue to be identified even after sequencing hundreds of genomes.