RECOMBINANT BOVINE INTERFERON-GAMMA AS AN IMMUNOMODULATOR IN DEXAMETHASONE-TREATED AND NONTREATED CATTLE
RECOMBINANT BOVINE INTERFERON-GAMMA AS AN IMMUNOMODULATOR IN DEXAMETHASONE-TREATED AND NONTREATED CATTLE
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DOI：
10.1089/jir.1989.9.143
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发表时间：
1989-02-01
期刊：
影响因子：
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通讯作者：
FRANK, DE
中科院分区：
文献类型：
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作者：
ROTH, JA;FRANK, DE
三种剂量（0.1、0.5和2.5 mg/只，皮下注射），重组牛干扰素- γ。（rBoIFN-. γ .）对牛中性粒细胞功能和淋巴细胞发生的体内影响进行了评价。三种给药剂量（0.5 mg/只或1.1次）均为最佳。106 U/只)，然后评估其对正常和地塞米松处理牛的中性粒细胞和淋巴细胞的影响。一只动物接受了2.5毫克的rBoIFN- γ。于给药后24小时死于急性弥漫性间质性肺炎伴小叶间水肿及肺气肿。使用的两种最高剂量在给药后24小时引起发烧，最高剂量在给药后24小时引起淋巴细胞囊胚发生减少。rBoIFN- γ的影响。嗜中性粒细胞功能是剂量依赖性的，并取决于嗜中性粒细胞功能的基线值。在接受0.5 mg或更多rBoIFN- γ的动物中，中性粒细胞的随机迁移一直受到抑制。rBoIFN- γ增强了金黄色葡萄球菌的摄入和中性粒细胞介导的抗体依赖性细胞毒性。地塞米松治疗牛和非地塞米松治疗牛的治疗，治疗前这些参数的值相对较低。中性粒细胞的碘化作用也被rBoIFN-. γ增强。当使用中性粒细胞兴奋剂浓度低于最佳浓度或用地塞米松治疗时。总之，rBoIFN-. γ。免疫抑制牛的免疫调节活性高于正常牛。rBoIFN- γ在体内的影响。因此取决于动物的生理状态。
Three dosages (0.1, 0.5, and 2.5 mg/animal, subcutaneously), of recombinant bovine interferon-.gamma. (rBoIFN-.gamma.) were evaluated for their in vivo influence on neutrophil function and lymphocyte blastogenesis in cattle. The optimal of the three dosages tested (0.5 mg/animal or 1.1 .times. 106 U/animal) was then evaluated for its influence on neutrophils and lymphocytes in both normal and dexamethasone-treated cattle. One animal, which received 2.5 mg of rBoIFN-.gamma., died by 24 h after administration due to acute diffuse interstitial pneumonia with interlobular edema and emphysema. The two highest dosages used caused fever at 24 h and the highest dosage caused a decrease in lymphocyte blastogenesis at 24 h after administration. The influence of rBoIFN-.gamma. on neutrophil function was dose dependent and depended on the baseline values for neutrophil function. Random migration by neutrophils was consistently inhibited in animals that received 0.5 mg or more of rBoIFN-.gamma.. Staphylococcus aureus ingestion and antibody-dependent cell-mediated cytotoxicity by neutrophils was enhanced by rBoIFN-.gamma. treatment in both dexamethasone-treated cattle and in nondexamethasone-treated cattle, which had relatively low values for these parameters before treatment. Iodination by neutrophils was also enhanced by rBoIFN-.gamma. when either a suboptimal concentration of neutrophil stimulant was used or when the cattle were treated with dexamethasone. In summary, the rBoIFN-.gamma. had greater immunomodulator activity in immunosuppressed than in normal cattle. The in vivo influence of rBoIFN-.gamma. therefore depends on the physiologic status of the animal.