Identification of a novel splicing isoform of murine CGI-58.
Identification of a novel splicing isoform of murine CGI-58.
复制标题
鼠 CGI-58 的新型剪接亚型的鉴定。
DOI：
10.1016/j.febslet.2009.12.058
复制
发表时间：
2010
期刊：
影响因子：
3.5
通讯作者：
Liu,Jun
中科院分区：
文献类型：
--
作者：
Yang,Xingyuan;Lu,Xin;Liu,Jun
比较基因识别 58 (CGI-58) 基因的突变与 Chanarin-Dorfman 综合征相关，编码 α/β 水解酶结构域亚家族的蛋白质。我们在此报告了小鼠 CGI-58 基因的一种新的选择性剪接亚型，称为 mCGI-58S。序列比较表明该 cDNA 变体缺乏第二和第三外显子。虽然全长蛋白显示出依赖周脂蛋白的脂滴定位，但 mCGI-58S 在细胞中表达时显示出主要的细胞质染色。 mCGI-58S 不能激活脂肪甘油三酯脂肪酶，但保留酰化溶血磷脂酸的能力。 mCGI-58S 的过表达未能促进脂滴周转和细胞内三酰甘油的损失。这些结果表明这种剪接事件可能参与脂质稳态的调节。
The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the α/β hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.