PROTEOLIPIDS .I. FORMATION OF PHOSPHOLIPID-CYTOCHROME C COMPLEXES
PROTEOLIPIDS .I. FORMATION OF PHOSPHOLIPID-CYTOCHROME C COMPLEXES
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DOI：
10.1021/bi00893a017
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发表时间：
1964-01-01
期刊：
影响因子：
2.9
通讯作者：
CRANE, FL
中科院分区：
文献类型：
--
作者：
DAS, ML;CRANE, FL
描述了细胞色素c与磷脂之间形成络合物的方法。根据形成条件，这些配合物接近一定的化学计量比例。当原始细胞色素c超过磷脂时，由混合磷脂形成的主要蛋白脂复合物每1个细胞色素c含有22克磷原子。当加入更多的混合磷脂时，比例接近32:1。醇加速了化学计量络合物的形成。一价阳离子对络合物形成的抑制作用与溶液的离子强度成正比，而二价和三价阳离子则完全抑制络合物的形成。有证据表明，混合磷脂产生一种复合物，其中与细胞色素c结合的磷的数量接近细胞色素c上可用的带电位点的数量。
A method is described for the formation of complexes between cytochrome c and phospholipids. These complexes are shown to approach certain stoichiometric proportions depending upon the conditions of formation. The major proteolipid complex formed from mixed phospholipids contains 22 gram atoms of phosphorus per 1 [image] of cytochrome c when the original cytochrome c is in excess of the phospholipid. When more mixed phospholipid is added the ratio approaches 32:1. Alcohol hastens the formation of the stoichiometric complex. Inhibition of complex formation by monovalent cations is proportional to ionic strength of the solution whereas di- and trivalent cations completely inhibit complex formation. The evidence indicates that mixed phospholipids produce a complex in which the amount of phosphorus bound to cytochrome c approaches the available number of charged sites on the cytochrome c.