Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies
Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies
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DOI：
10.1016/j.eururo.2008.04.043
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发表时间：
2009-07-01
期刊：
影响因子：
23.4
通讯作者：
Montironi, Rodolfo
中科院分区：
文献类型：
--
作者：
Barbisan, Francesca;Mazzucchelli, Roberta;Montironi, Rodolfo
背景：人类 ELAV 样蛋白 HuR 调节多个 mRNA 靶标的稳定性，包括 cyclooygenase-2 (COX-2)。它们在前列腺癌发生中的表达尚不确定。目的：分析偶发性前列腺癌膀胱前列腺切除术（CyPs）中HuR和COX-2的表达，并将其与临床检测出癌症的根治性前列腺切除术（RPs）中的表达进行比较。设计、背景和参与者：在正常上皮（NEp）、萎缩、高级别前列腺上皮内瘤变中对HuR和COX-2进行免疫组织化学评估(HGPIN) 和前列腺癌 (PCa) 在 20 个 CyP 和 20 个 RP 中，这两种类型的 pT2a 格里森评分为 6 PCa 的标本。测量：在每个病例中，在连续 400X 显微镜视野中至少计数 1000 个细胞，分别针对 NEp、萎缩、HGPIN 和 PCa。结果和局限性：具有胞质 HuR 的分泌细胞的百分比有所增加从NEp到萎缩的染色。 HGPIN 和 PCa。 CyP 和 RP 中，与 PCa 相邻的 NEp、萎缩和 HGPIN 的平均百分比均大于远离癌症的百分比。萎缩中 HGPIN 存在核 HuR 表达减少的趋势。和 PCa，与 NEp 相比。在基底细胞和分泌细胞的细胞质中可见COX-2染色。与 NEp 相比，阳性基底细胞的平均比例有所减少，而从萎缩到 HGPIN 和 PCa 的阳性分泌细胞的百分比逐渐增加。细胞质 HuR 过表达与 COX-2 表达相关。肿瘤体积为0.5 ccm的癌症之间HuR和COX-2的表达没有差异。本研究的局限性是调查的病例数较少且缺乏无癌症的对照组。结论：分泌细胞的HuR染色从NEp的细胞核转移到PCa的细胞质。这与 COX-2 表达从基底细胞到分泌细胞的平行转变有关。 (C) 2008 年欧洲泌尿外科协会。由 Elsevier B.V. 出版。保留所有权利。
Background: The human ELAV-like protein HuR regulates the stability of several mRNA targets, including that of cyclooygenase-2 (COX-2). Their expression in prostatic carcinogenesis is uncertain.Objective: To analyze HuR and COX-2 expression in cystoprostatectomies (CyPs) with incidental prostate cancer and compare their expression with those in radical prostatectomies (RPs) with clinically detected cancer.Design, setting, and participants: HuR and COX-2 were immunohistochemically evaluated in normal-looking epithelium (NEp), atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN), and prostate carcinoma (PCa) in 20 CyPs and 20 RPs, both types of specimens with pT2a Gleason score 6 PCa.Measurements: At least 1000 cells were counted in contiguous 400X microscopic fields in each case, separately for NEp, atrophy, HGPIN, and PCa.Results and limitations: There was an increase in the percentage of secretory cells with cytoplasmic HuR staining from NEp to atrophy. HGPIN, and PCa. The mean percentages in NEp, atrophy, and HGPIN adjacent to PCa were greater than away from cancer, both in the CyP and RPs. There was a trend towards a reduced nuclear HuR expression in atrophy, HGPIN. and PCa, compared to NEp. COX-2 staining was seen in the cytoplasm of the basal and secretory cells. There was a reduction in the mean Proportion of positive basal cells and progressive increase in the percentage of positive secretory cells from atrophy to HGPIN and PCa, compared to NEp. Cytoplasmic HuR overexpression was correlated with COX-2 expression. There was no difference in HuR and COX-2 expression between cancers with tumour volume 0.5 ccm.The limitations of this study were the small number of cases investigated and lack of a control group without cancer.Conclusions: The secretory cells showed shift in HuR staining from nuclear in NEp to cytoplasmic in PCa. This is associated with a parallel shift in COX-2 expression from basal to secretory cells. (C) 2008 European Association of Urology. Published by Elsevier B.V. All rights reserved.