Microfabrication of single-use plastic microfluidic devices for high-throughput screening and DNA analysis
Microfabrication of single-use plastic microfluidic devices for high-throughput screening and DNA analysis
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DOI：
10.1007/s005420100114
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Schaller, T
中科院分区：
文献类型：
--
作者：
Gerlach, A;Knebel, G;Schaller, T
现代药物发现和基因组分析依赖于大量样本的并行快速分析。微流控器件在这一领域的适用性需要低成本的器件，这些器件可以大规模生产。在密切合作下，Greiner Bio-One和Forschungszentum Karlsruhe开发了一种在标准化微板足迹中一次性使用的塑料微流控毛细管电泳(CE)阵列。可行性研究表明，用机械微机械成型工具进行热压印是低成本大规模制造的合适技术。微通道的后续密封允许96通道多路微结构中的亚微升样品体积。
Modern drug discovery and genomic analysis depend on rapid analysis of large numbers of samples in parallel. The applicability of microfluidic devices in this field needs low cost devices, which can be fabricated in mass production. In close collaboration, Greiner Bio-One and Forschungszentrum Karlsruhe have developed a single-use plastic microfluidic capillary electrophoresis (CE) array in the standardized microplate footprint. Feasibility studies have shown that hot embossing with a mechanical micromachined molding tool is the appropriate technology for low cost mass fabrication. A subsequent sealing of the microchannels allows sub-microliter sample volumes in 96-channel multiplexed microstructures.