Soybean Allergens Affecting North American Patients Identified by 2D Gels and Mass Spectrometry
Soybean Allergens Affecting North American Patients Identified by 2D Gels and Mass Spectrometry
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DOI：
10.1007/s12161-009-9090-3
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发表时间：
2010-12-01
影响因子：
2.9
通讯作者：
Gleddie, Stephen
中科院分区：
文献类型：
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作者：
Gagnon, Christine;Poysa, Vaino;Gleddie, Stephen
在这项研究中，我们比较了免疫球蛋白E的免疫反应23大豆过敏，9大豆敏感，和4个非过敏的人血清大豆蛋白分离的1D和2D凝胶电泳和Western印迹筛选。这种方法导致了新的过敏原鉴定大豆蛋白的串联质谱分析反应蛋白。大豆是引起北美人过敏反应的八大食物之一。以前的研究已经确定了大豆种子中存在的几种假定的过敏原;然而，这些报告中的许多没有采用质谱法来最终确定过敏蛋白。有评论认为，大豆种子可能含有10到20种不同的蛋白质，这些蛋白质会引起敏感患者的过敏反应。在这项对北美患者的研究中，我们报告了总共19种潜在的过敏原蛋白，包括10种通过质谱鉴定的蛋白和5种新的过敏原。我们还广泛使用了缺乏主要种子贮藏蛋白、大豆球蛋白和β-伴大豆球蛋白的各种亚基的大豆品系。通过在患者血清的蛋白质印迹中使用这些敲除系，最终证明一些患者主要仅对少数蛋白质反应，而大多数患者对4至6种蛋白质反应。本文的研究结果描述了大豆种子中存在的主要过敏蛋白，这些过敏原在北美人中的相对重要性，以及一些缺乏个别过敏原的大豆遗传系。
In this study, we compared the immunoglobulin E immunological reactions of 23 soy-allergic, nine soy-sensitive, and four non-allergic human sera to soybean proteins separated by 1D and 2D gel electrophoresis and screened by Western blot. This method led to novel allergen identification in soybean proteins by tandem mass spectrometry analysis of reactive proteins. Soybean is one of the eight most significant foods which provoke allergic reactions among North Americans. Previous studies have identified several putative allergens present in soybean seeds; however, many of these reports did not employ mass spectrometry to conclusively identify the allergenic proteins. Reviews have suggested that soybean seeds may contain between 10 and 20 different proteins responsible for provoking allergic reactions among sensitive patients. We report, in this study of North American patients, a total of 19 potentially allergenic proteins including ten identified by mass spectrometry and five novel allergens. We have also made extensive use of soybean lines lacking various subunits of the major seed storage proteins, glycinin and beta-conglycinin. By using these knockout lines in Western blots with patient serum, it was conclusively demonstrated that some patients react predominantly to only a few proteins, while most react to four to six proteins. The findings herein describe the main allergic proteins present in soybean seeds, the relative significance of these allergens among North Americans, and some genetic lines of soybean lacking individual allergens.