Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10
Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10
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嗜肺军团菌血清群 10 引起的院内肺炎的宏观限制分析和单克隆抗体流行病学调查
DOI：
10.1128/jcm.32.11.2692-2697.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
Maria Klepp
中科院分区：
文献类型：
--
作者：
P. Christian;Luck;J. Helbig;'. U. Gunter;Michael Assmann;Ralf Blau;'. H. Koch;Maria Klepp
一名 67 岁女性因左下叶急性肺炎住院。从第 18 天和第 20 天采集的两份痰标本中培养嗜肺军团菌血清群 10，并使用市售的物种特异性单克隆抗体以及血清群 10 特异性单克隆抗体通过直接免疫荧光测定进行检测。使用血清群 10 特异性多克隆和单克隆抗体在酶联免疫吸附测定中检测抗原尿。在间接免疫荧光测试中，血清中发现针对血清群 1、4、5、8、9、10、14 和 15 的抗体滴度上升，其中针对血清群 8、9 和 10 的抗体滴度最高。嗜肺军团菌血清群 10 和 6 以及与血清群 4 和 14 抗血清反应的菌株从两个中心培养及医院周边热水系统。通过脉冲场凝胶电泳对基因组 DNA 进行宏观限制分析表明，从患者身上分离出的菌株与医院热水系统中的血清群 10 菌株相同。相比之下，16 个不相关的嗜肺军团菌血清群 10 菌株的基因组 DNA 显示出 12 种不同的限制模式。单克隆抗体亚型分析显示，从不同来源分离的嗜肺军团菌血清组 10 菌株仅存在微小差异。总之，宏观限制分析是研究嗜肺军团菌血清群 10 分子流行病学的一个有价值的工具。
A 67-year-old woman was hospitalized with an acute pneumonia of the left lower lobe. Legionella pneumophila serogroup 10 was cultured from two sputum specimens taken on days 18 and 20 and was also detected by direct immunofluorescence assay by using a commercially available species-specific monoclonal antibody as well as serogroup 10-specific monoclonal antibodies. Antigenuria was detected in enzyme-linked immunosorbent assays by using serogroup 10-specific polyclonal and monoclonal antibodies. In the indirect immunofluorescence test rising antibody titers against serogroups 1, 4, 5, 8, 9, 10, 14, and 15 were found in serum, with the highest titers found against serogroups 8, 9, and 10. L. pneumophila serogroups 10 and 6 and a strain that reacted with serogroup 4 and 14 antisera were cultured from both central and peripheral hot water systems of the hospital. Macrorestriction analyses of the genomic DNAs by pulsed-field gel electrophoresis showed that the isolate from the patient was identical to the serogroup 10 strains from the hospital hot water system. In contrast, the genomic DNAs of 16 unrelated L. pneumophila serogroup 10 strains showed 12 different restriction patterns. Monoclonal antibody subtyping revealed only minor differences in L. pneumophila serogroup 10 strains isolated from different sources. In conclusion, macrorestriction analysis is a valuable tool for studying the molecular epidemiology of L. pneumophila serogroup 10.