Dynamic and reversible fluorescence imaging of superoxide anion fluctuations in live cells and in vivo.
Dynamic and reversible fluorescence imaging of superoxide anion fluctuations in live cells and in vivo.
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DOI：
10.1021/ja408524j
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发表时间：
2013-09
影响因子：
15
通讯作者：
Wen Zhang;Ping Li;Fan Yang;Xiufen Hu;Chuanzhi Sun;Wei Zhang;Dezhan Chen;Bo Tang
中科院分区：
文献类型：
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作者：
Wen Zhang;Ping Li;Fan Yang;Xiufen Hu;Chuanzhi Sun;Wei Zhang;Dezhan Chen;Bo Tang
活性氧（ROS）的过度产生与细胞损伤和疾病密切相关。由于超氧阴离子（O2（·-））是其它活性氧的前体，因此研究O2（·-）在细胞内和体内的波动具有重要意义。为了解决这一关键需求，我们开发了一种具有单光子和双光子荧光特性的新型可逆荧光探针，该探针非常适合选择性和动态地监测O2（·-）通量。成像结果证实了该探针在细胞凋亡刺激下对细胞内O2（·-）的动态和可逆的荧光响应。此外，该探针可以方便地可视化在肝细胞，斑马鱼和小鼠的再灌注损伤过程中的O2（·-）浓度的变化，通过单光子或双光子成像根据不同的样品的深度。本研究提供了一个强大的荧光成像工具的动态跟踪O2（·-）在活细胞和体内。
Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O2(•-)) is the precursor of other ROS, exploring O2(•-) fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O2(•-) fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O2(•-) under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O2(•-) concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O2(•-) in live cells and in vivo.