Alternate polyadenylation in human mRNAs: A large-scale analysis by EST clustering
Alternate polyadenylation in human mRNAs: A large-scale analysis by EST clustering
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DOI：
10.1101/gr.8.5.524
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发表时间：
1998-05-01
期刊：
影响因子：
7
通讯作者：
Claverie, JM
中科院分区：
文献类型：
--
作者：
Gautheret, D;Poirot, O;Claverie, JM
交替多聚腺苷酸化是所有重要的转录后调控过程，现在可以利用cDNA数据库进行大规模分析。我们将164，000个表达序列标签（EST）聚类成类似于15，000个组，并将每组与推定的mRNA 3'端进行比对。通过使用严格的标准，以丢弃人为的mRNA的四肢，明确的证据，交替聚腺苷酸化，获得了189的1000个EST簇的研究。一些以前未报道的多聚腺苷酸化位点被确定，连同组织特异性差异多聚腺苷酸化的可能情况。这项研究表明，除了基因表达的定量方面，交替mRNA形式的分布可以通过EST采样进行分析。
Alternate polyadenylation is all important post-transcriptional regulatory process now open to large-scale analysis by use of cDNA databases. We clustered 164,000 expressed sequence tags (ESTs) into similar to 15,000 groups and aligned each group to a putative mRNA 3' end. By use of stringent criteria to discard artifactual mRNA extremities, clear evidence for alternate polyadenylation was obtained in 189 of the 1000 EST clusters studied. A number of previously unreported polyadenylation sites were identified, together with possible instances of tissue-specific differential polyadenylation. This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.