Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice
Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice
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DOI：
10.1007/s00438-008-0406-6
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发表时间：
2009-02-01
影响因子：
3.1
通讯作者：
Ashikari, Motoyuki
中科院分区：
文献类型：
--
作者：
Asano, Kenji;Hirano, Ko;Ashikari, Motoyuki
sd 1被称为水稻中的“绿色革命”基因，因为它在水稻育种中的应用显著提高了水稻产量。自"绿色革命“以来，sd 1已被广泛用于生产现代半矮秆品种。然而，有限矮源的广泛应用可能会在水稻品种的遗传背景中产生瓶颈效应。为了解决这个问题，必须确定新的和有用的来源的矮秆基因。在这项研究中，我们确定了三个半显性矮秆突变体。根据节间伸长模式，这些突变体被归类为dn-type矮秆突变体。赤霉素（GA）的反应测试表明，突变体仍然响应GA，但在降低的速度。基于图位的克隆显示，这些突变体的矮化表型是由SLR 1的N-末端区域的功能获得性突变引起的。这些突变体中SLR 1蛋白的降解发生晚于野生型。使用酵母双杂交系统也观察到这些突变体中SLR 1蛋白与GID 1的相互作用能力降低。杂交试验表明，在适当的遗传背景下，本研究所鉴定的半显性矮秆等位基因可用于缓解矮秆基因的不足。
sd1 is known as the 'green revolution' gene in rice because its application in rice breeding has dramatically increased rice yield. Since the 'green revolution,' sd1 has been extensively used to produce modern semi-dwarf varieties. The extensive use of limited dwarfing sources may, however, cause a bottleneck effect in the genetic background of rice varieties. To circumvent this problem, novel and useful sources of dwarf genes must be identified. In this study, we identified three semi-dominant dwarf mutants. These mutants were categorized as dn-type dwarf mutants according to the elongation pattern of internodes. Gibberellin (GA) response tests showed that the mutants were still responsive to GA, although at a reduced rate. Map-based cloning revealed that the dwarf phenotype in these mutants was caused by gain-of-function mutations in the N-terminal region of SLR1. Degradation of the SLR1 protein in these mutants occurred later than in the wild type. Reduced interaction abilities of the SLR1 protein in these mutants with GID1 were also observed using the yeast two-hybrid system. Crossing experiments indicated that with the use of an appropriate genetic background, the semi-dominant dwarf alleles identified in this study could be used to alleviate the deficiency of dwarfing genes for breeding applications.