Universal sample preparation method for proteome analysis
Universal sample preparation method for proteome analysis
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DOI：
10.1038/nmeth.1322
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发表时间：
2009-05-01
期刊：
影响因子：
48
通讯作者：
Mann, Matthias
中科院分区：
文献类型：
--
作者：
Wisniewski, Jacek R.;Zougman, Alexandre;Mann, Matthias
我们描述了一种方法，过滤辅助样品制备（FASP），它结合了凝胶内和溶液内消化的优点，用于基于质谱的蛋白质组学。我们将蛋白质组完全溶解在十二烷基硫酸钠中，然后在标准过滤装置上用尿素交换。在过滤器上消化后洗脱的肽是纯的，允许细胞器的单次分析和前所未有的蛋白质组覆盖深度。
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.