High JC virus load in gastric cancer and adjacent non-cancerous mucosa
High JC virus load in gastric cancer and adjacent non-cancerous mucosa
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DOI：
10.1111/j.1349-7006.2006.00354.x
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发表时间：
2007-01-01
期刊：
影响因子：
5.7
通讯作者：
Takano, Yasuo
中科院分区：
文献类型：
--
作者：
Murai, Yoshihiro;Zheng, Hua-Chuan;Takano, Yasuo
JC病毒（JCV）感染了世界上很大一部分人口，大约90%的成年人血清呈阳性。最近的报道描述了它在几种恶性肿瘤中的致癌作用的可能性。本研究的目的是评估JCV对胃癌的致癌意义。采用巢式聚合酶链反应（PCR）、Southern印迹法、DNA直接测序法、实时荧光定量PCR法、原位荧光定量PCR法和免疫组化法，对22对新鲜肿瘤及癌旁非癌组织（ANCT）和10例正常胃粘膜标本进行了研究。T抗原序列在86.4%的胃癌和ANCT中检测到，在100%的正常粘膜样品中检测到，病毒衣壳蛋白分别为54.1%，68.1%和70%。agnoprotein的发病率一般较低。胃癌和配对ANCT的JCV DNA负荷（分别为4784 +/- 759和5394 +/- 1466拷贝/ μ g DNA）比正常胃组织（542.4 +/- 476.0拷贝/ μ g DNA， P < 0.0001）高约10倍。原位PCR显示散发性JCV基因组阳性的癌细胞和中央凹上皮细胞。免疫组化检测T抗原蛋白表达仅1例（1/22;4.5%），可能是由于T抗原半衰期较短。结论：大多数日本人胃上皮感染JCV的比例较低，但在癌细胞和ANCT中感染水平均显著升高，表明JCV拷贝的增殖可能是胃癌发生的危险因素和背景。
The JC virus (JCV) infects a large proportion of the worldwide population and approximately 90% of adults are seropositive. Recent reports have described the possibility of its oncogenetic role in several malignancies. The aim of the present study was to assess the oncogenetic significance of JCV for gastric cancer. Twenty-two sample pairs of fresh tumor and adjacent non-cancerous tissue (ANCT) as well as 10 normal gastric mucosa specimens were investigated on the basis of nested polymerase chain reaction (PCR) followed by Southern blotting, DNA direct sequencing, real-time PCR, in situ PCR and immunohistochemistry. The T antigen sequence was detected in 86.4% of gastric cancers and ANCT, and in 100% of the normal mucosa samples, as for virus capsid protein, 54.1%, 68.1% and 70%, respectively. A generally low incidence was noted for agnoprotein. The JCV DNA load was approximately 10-fold higher in both gastric cancers and paired ANCT (4784 +/- 759 and 5394 +/- 1466 copies/mu g DNA, respectively) than in normal gastric tissue (542.4 +/- 476.0 copies/mu g DNA, P < 0.0001). In situ PCR revealed sporadic JCV genome-positive cancer cells and foveolar epithelial cells. T antigen protein expression assessed by immunohistochemistry was detected only in one case (1/22; 4.5%), probably because the half life of T antigen might be short. It was concluded that the gastric epithelium in most Japanese people is infected with JCV at a low rate but levels of infection are increased markedly in both cancer cells and ANCT, indicating that multiplication of JCV copies might be a risk factor and a background for gastric carcinogenesis.