MOLECULAR PHYLOGENY OF THE GRASS GENUS BRACHYPODIUM P-BEAUV BASED ON RFLP AND RAPD ANALYSIS
MOLECULAR PHYLOGENY OF THE GRASS GENUS BRACHYPODIUM P-BEAUV BASED ON RFLP AND RAPD ANALYSIS
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DOI：
10.1006/bojl.1995.0018
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发表时间：
1995-04-01
影响因子：
2.4
通讯作者：
STACE, CA
中科院分区：
文献类型：
--
作者：
CATALAN, P;SHI, Y;STACE, CA
利用RFLP和RAPD技术对短柄草属不同种进行了核基因组分析。和其他禾本科植物的代表，以确定Brachypodium基因组的特征，并建立其相对于其他Pooideae的进化位置。短柄草基因组的显著特征是其小尺寸、低重复DNA量、测定探针/酶组合的属内缺乏限制性片段长度多态性以及通过随机DNA扩增在物种水平上证明的基因组变异性。这些分子生物学研究证实Brachypodium是一个孤立的古老属，最好放在自己的部落（Brachypodieae）。它的关系，以其他部落Bromeae，小麦，Poeae解决，Brachypodieae是最早的部落，从这个核心的pooids。在该属内，两个主要的旧大陆分支是可区分的：一年生分支，仅由B代表。二穗型;和多年生分支，所研究的所有其他种（除了B。mexicanum）。多年生美洲种B。mexicanum似乎同样依附于这两个分支。RFLP数据被认为是有用的，在获得属和更高的等级水平的遗传，而高度可变的RAPD数据更适合于解决种间和种内的进化途径。
Nuclear genome analysis using RFLPs and RAPDs has been assessed within different species of the genus Brachypodium P. Beauv. and representatives of other grasses in order to determine the characteristics of the Brachypodium genome and to establish its evolutionary position in relation to other Pooideae. Distinctive features of the Brachypodium genome are its small size, the low amount of repetitive DNA, the lack of restriction fragment length polymorphisms within the genus for the assayed probe/enzyme combinations, and the genomic variability demonstrated at species level by random DNA amplification. These molecular studies confirm Brachypodium as an isolated ancient genus best placed in its own tribe (Brachypodieae). Its relationships to other tribes-Bromeae, Triticeae, Poeae-are resolved, Brachypodieae being the earliest tribe to diverge from this core of pooids. Within the genus two major Old World clades are distinguishable: an annual clade, represented only by B. distachyon; and a perennial clade, represented by all the other species studied (except B. mexicanum). The perennial American species B. mexicanum appears equally attached to these two clades. RFLP data were found to be useful in obtaining phylogenies at generic and higher rank levels, whereas the highly variable RAPD data were more suitable for resolving interspecific and intraspecific evolutionary pathways.