Plasmids harboring a tandem duplicate of blaVIM-24 in carbapenem-resistant ST1816 Pseudomonas aeruginosa in Japan
Plasmids harboring a tandem duplicate of blaVIM-24 in carbapenem-resistant ST1816 Pseudomonas aeruginosa in Japan
复制标题
日本耐碳青霉烯类 ST1816 铜绿假单胞菌中含有 blaVIM-24 串联副本的质粒
DOI：
10.1089/mdr.2022.0168
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发表时间：
2023
影响因子：
2.6
通讯作者：
Kirikae Teruo
中科院分区：
文献类型：
--
作者：
Hishinuma Tomomi;Tada Tatsuya;Tohya Mari;Shintani Masaki;Suzuki Masato;Shimojima Masahiro;Kirikae Teruo
本研究旨在阐明铜绿假单胞菌ST 1816分离株中blaVIM-24串联重复的生物学和临床意义。2016年至2019年期间，从日本的两个医疗机构获得了13株携带blaVIM质粒的ST 1816分离株。完全测序显示，在13个质粒中，有4个具有blaVIM-24的串联重复。这四种质粒分别含有属于IncP-9、MOBF和MPFT的复制子、松弛酶基因和T4 SS基因。所有四种质粒均通过过滤器交配转移至PAO 1。头孢吡肟对PAO 1的生长影响很小，PAO 1携带一个含有串联重复的pUCP 19。Western blotting分析表明，含有串联拷贝的PAO 1酶产生的Vim-24金属β-内酰胺酶的相对强度是含有单拷贝的PAO 1酶产生的VIM-24金属β-内酰胺酶的2.6倍。这些结果表明，blaVIM-24在质粒中的串联重复可能赋予对头孢吡肟的抗性，从而使P. aerobiosaST 1816菌株在日本的医院中增殖。
The aim of this study was to clarify the biological and clinical significance of a tandem duplicate ofblaVIM-24inPseudomonas aeruginosaST1816 isolates. Thirteen ST1816 isolates carrying a plasmid harboringblaVIMswere obtained from two medical settings in Japan between 2016 and 2019. Complete sequencing revealed that, of the 13 plasmids, four had a tandem duplicate ofblaVIM-24. These four plasmids harbored a replicon, a relaxase gene, and T4SS genes belonging to IncP-9, MOBF, and MPFT, respectively. All four plasmids transferred to PAO1 by filter mating. Cefepime marginally affected the growth of PAO1, carrying a pUCP19 harboring the tandem duplicate. Western blotting analysis showed that the relative intensity of VIM-24 metallo-β-lactamase produced by a PAO1 transformant containing a tandem duplicate was 2.6-fold higher than that produced by a PAO1 transformant containing a single copy. These results suggest that the tandem duplicate ofblaVIM-24in plasmids may confer resistance against cefepime, enablingP. aeruginosaST1816 strains to proliferate in hospitals in Japan.