Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity
Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity
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DOI：
10.1111/j.1365-2672.2007.03433.x
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发表时间：
2007-11-01
影响因子：
4
通讯作者：
Bloemberg, G.
中科院分区：
文献类型：
--
作者：
Cazorla, F. M.;Romero, D.;Bloemberg, G.
目的：本研究旨在从鳄梨根面分离出具有抗土传植物病原真菌生物活性的枯草芽孢杆菌菌株。方法和结果：从健康鳄梨树的根面获得的 905 个细菌分离株，包含 277 个革兰氏阳性分离株。从这些革兰氏阳性分离株中，根据其对多种土传植物病原真菌的抗真菌活性，选择了四种菌株：PCL1605、PCL1608、PCL1610 和 PCL1612，被鉴定为枯草芽孢杆菌。对参与其拮抗活性的抗真菌化合物的分析表明，这些菌株产生水解酶，例如葡聚糖酶或蛋白酶以及抗生素脂肽表面活性素、风霉素和/或伊图林A。在使用番茄/尖孢镰刀菌病害系统的生物防治试验中。 radicis-lycopersici 和 avocado/Rosellinia necatrix 中，两种枯草芽孢杆菌菌株 PCL1608 和 PCL1612 均产生伊曲霉素 A，表现出最高的生防和定植能力。结论：从健康牛油果根面分离的多种拮抗性枯草芽孢杆菌菌株显示出良好的生防能力， 与抗真菌脂肽的产生和良好的定植能力密切相关。该研究的意义和影响：这是涉及对常见土传植物病原真菌尖孢镰刀菌专化型（F. oxysporum f.sp）具有生物防治活性的枯草芽孢杆菌菌株的分离和表征的少数报告之一。 radicis-lycopersici 和 R. necatrix。
Aims: This study was undertaken to isolate Bacillus subtilis strains with biological activity against soil-borne phytopathogenic fungi from the avocado rhizoplane.Methods and Results: A collection of 905 bacterial isolates obtained from the rhizoplane of healthy avocado trees, contains 277 gram-positive isolates. From these gram-positive isolates, four strains, PCL1605, PCL1608, PCL1610 and PCL1612, identified as B. subtilis, were selected on the basis of their antifungal activity against diverse soil-borne phytopathogenic fungi. Analysis of the antifungal compounds involved in their antagonistic activity showed that these strains produced hydrolytic enzymes such as glucanases or proteases and the antibiotic lipopeptides surfactin, fengycin, and/or iturin A. In biocontrol trials using the pathosystems tomato/Fusarium oxysporum f.sp. radicis-lycopersici and avocado/Rosellinia necatrix, two B. subtilis strains, PCL1608 and PCL1612, both producing iturin A, exhibited the highest biocontrol and colonization capabilities.Conclusions: Diverse antagonistic B. subtilis strains isolated from healthy avocado rhizoplanes have shown promising biocontrol abilities, which are closely linked with the production of antifungal lipopeptides and good colonization aptitudes.Significance and Impact of the Study: This is one of the few reports dealing with isolation and characterization of B. subtilis strains with biocontrol activity against the common soil-borne phytopathogenic fungi F. oxysporum f.sp. radicis-lycopersici and R. necatrix.