ACTIVATION OF HUMAN LYMPHOCYTES-B THROUGH CD40 AND INTERLEUKIN-4
ACTIVATION OF HUMAN LYMPHOCYTES-B THROUGH CD40 AND INTERLEUKIN-4
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DOI：
10.1002/eji.1830190818
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发表时间：
1989-08-01
影响因子：
5.4
通讯作者：
BANCHEREAU, J
中科院分区：
文献类型：
--
作者：
VALLE, A;ZUBER, CE;BANCHEREAU, J
我们已经产生并表征了一种新的CD 40单克隆抗体，mAb 89，其在抗IgM抗体的存在下共刺激诱导B细胞增殖。mAb 89激活静息B细胞，如细胞体积增加和随后响应抗IgM抗体的B细胞增殖增强所示。然而，mAb 89不能使B细胞对白细胞介素（IL）2或IL 4的促生长活性产生应答。与IL 2和IL 4不同，mAb 89仅微弱地刺激抗IgM预活化的B细胞的增殖。因此，抗-CD 40的活化特性可能解释其对B细胞的共刺激作用。有趣的是，在共刺激和再刺激试验中，发现抗CD 40 mAb 89与IL 4协同作用，但与IL 2不协同作用。在这方面，抗-CD 40不诱导B细胞表面IL 4受体的显著增加，而IL 4而不是IL 2诱导CD 40抗原表达的两倍增加。因此，IL 4与抗CD 40之间的协同作用可能与IL 4依赖性的CD 40抗原表达增加有关。
We have produced and characterized a new CD40 monoclonal antibody, mAb 89, which in the presence of anti-IgM antibodies co-stimulates to induce B cell proliferation. mAb 89 activates resting B cells as shown by an increase in cell volume and an enhanced subsequent proliferation of B cells in response to anti-IgM antibody. However, mAb 89 does not prepare B cells to respond to the growth-promoting activity of interleukin (IL) 2 or IL 4. Unlike IL 2 and IL 4, mAb 89 only weakly stimulates the proliferation of anti-IgM pre-activated B cells. Thus, the activating properties of anti-CD40 are likely to explain its co-stimulatory effect on B cells. Interestingly, the anti-CD40 mAb 89 was found to act in synergy with IL 4, but not with IL 2 in co-stimulation and restimulation assays. In this respect, anti-CD40 does not induce a significant increase of B cell surface IL 4 receptors while IL 4, but not IL 2 induces a twofold increase of the CD40 antigen expression. Thus the synergistic interaction between IL 4 and anti-CD40 may be related to the IL 4-dependent increase of CD40 antigen expression.