Genomic profiling of renal cell carcinoma in patients with end-stage renal disease
Genomic profiling of renal cell carcinoma in patients with end-stage renal disease
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DOI：
10.1111/j.1349-7006.2011.02176.x
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发表时间：
2012-03-01
期刊：
影响因子：
5.7
通讯作者：
Moriyama, Masatsugu
中科院分区：
文献类型：
--
作者：
Inoue, Toru;Matsuura, Keiko;Moriyama, Masatsugu
本研究的目的是通过分析基因组拷贝数畸变来确定终末期肾脏疾病（ESRD）肾细胞癌（RCC）的基因组谱。使用Agilent全人类基因组4 × 44K Oligo微阵列（Agilent Technologies Inc., Palo Alto， CA， USA）对63例RCC-ESRD患者的79份肿瘤样本进行阵列比较基因组杂交分析。无监督分层聚类分析结果显示，63例病例可分为A、B两组。A组以透明细胞RCC （CCRCC）为主，B组以乳头状RCC （PRCC）、获得性囊性疾病（ACD）相关RCC和透明细胞乳头状RCC为主。平均频率分析显示，聚类A和聚类B的基因组图谱分别与散发性CCRCC和散发性PRCC相似。尽管在组织病理学的基础上提出acd相关的RCC、透明细胞乳头状RCC和PRCC-ESRD是不同的亚型，但目前的数据显示，这些类型的基因组图谱被归类为B类，彼此相似。此外，PRCC、acd相关RCC和透明细胞乳头状RCC混合在一个组织中的基因组图谱趋于相似。基于RCC-ESRD的基因组分析，我们认为CCRCC- esrd的分子发病机制与散发性CCRCC相似。尽管非透明细胞RCC-ESRD有多种组织学亚型，但它们的基因组谱与散发性PRCC相似，这表明非ccrcc - esrd的分子发病机制可能与散发性PRCC有关。（癌症科学2012；103:569576）
The purpose of the present study was to determine the genomic profile of renal cell carcinoma (RCC) in end-stage renal disease (ESRD) by analyzing genomic copy number aberrations. Seventy-nine tumor samples from 63 patients with RCC-ESRD were analyzed by array comparative genomic hybridization using the Agilent Whole Human Genome 4 x 44K Oligo Micro Array (Agilent Technologies Inc., Palo Alto, CA, USA). Unsupervised hierarchical clustering analysis revealed that the 63 cases could be divided into two groups, Clusters A and B. Cluster A was comprised mainly of clear cell RCC (CCRCC), whereas Cluster B was comprised mainly of papillary RCC (PRCC), acquired cystic disease (ACD)-associated RCC, and clear cell papillary RCC. Analysis of the averaged frequencies revealed that the genomic profiles of Clusters A and B resembled those of sporadic CCRCC and sporadic PRCC, respectively. Although it has been proposed on the basis of histopathology that ACD-associated RCC, clear cell papillary RCC and PRCC-ESRD are distinct subtypes, the present data reveal that the genomic profiles of these types, categorized as Cluster B, resemble one another. Furthermore, the genomic profiles of PRCC, ACD-associated RCC and clear cell papillary RCC admixed in one tissue tended to resemble one another. On the basis of genomic profiling of RCC-ESRD, we conclude that the molecular pathogenesis of CCRCC-ESRD resembles that of sporadic CCRCC. Although various histologic subtypes of non-clear cell RCC-ESRD have been proposed, their genomic profiles resemble those of sporadic PRCC, suggesting that the molecular pathogenesis of non-CCRCC-ESRD may be related to that of sporadic PRCC. (Cancer Sci 2012; 103: 569576)