A STUDY OF AN INVITRO MODEL FOR INVASION OF ORAL SQUAMOUS-CELL CARCINOMA
A STUDY OF AN INVITRO MODEL FOR INVASION OF ORAL SQUAMOUS-CELL CARCINOMA
复制标题
DOI：
10.1111/j.1600-0714.1989.tb01350.x
复制
发表时间：
1989-10-01
影响因子：
3.3
通讯作者：
ENOMOTO, S
中科院分区：
文献类型：
--
作者：
MATSUMOTO, K;HORIKOSHI, M;ENOMOTO, S
为研究口腔鳞状细胞癌(SCC)的侵袭机制，用胶原凝胶与人成纤维细胞组成的特异性培养基质建立了口腔鳞癌体外侵袭机制模型。在单纯胶原凝胶上培养的5个SCC细胞系在凝胶上呈层状生长。然而，当在含有成纤维细胞的胶原凝胶上培养时，所有五种细胞系都显示出侵袭性生长到基质中。此外，成纤维细胞条件培养液还能促进HSC-3细胞向胶原凝胶的侵袭。提示成纤维细胞在口腔鳞状细胞癌体外侵袭中起重要作用。用该检测系统对实验室新建立的4株细胞进行了检测。体外培养的细胞系在含成纤维细胞的胶原凝胶上表现出与体内相似的形态和生物学特征。
An in vitro model for studying the invasion mechanism of oral squamous cell carcinoma (SCC) was developed using a specific culture matrix composed of collagen gel combined with human fibroblasts. Five SCC cell lines cultured on collagen-only gels showed stratified growth on the gels. However, all five cell lines showed invasive growth into the matrix when cultured on the fibroblast-incorporated collagen gels. Moreover, fibroblast-conditioned medium was shown to promote the invasion of HSC-3 cells into the collagen gels. These results suggest that fibroblasts play an important role in the invasion of oral SCC cells in vitro. Four cell lines, which were newly established in our labortory, were tested in this assay system. These cell lines cultured on fibroblast-incorporated collagen gels expressed morphologic and biologic characteristics in vitro, similar to those in vivo.