Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley.
Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley.
复制标题
聚合酶链式反应介导 RFLP 克隆定位到微分离的大麦易位染色体。
作者：
A. Sorokin;F. Marthe;A. Houben;U. Pich;A. Graner;G. Künzel
一种新的策略已被设计和用于物理定位的遗传映射的限制性片段长度多态性（RFLP）克隆大麦染色体。从同步化根尖分生组织中分离出形态不同的易位染色体，并将其DNA用作序列特异性引物进行聚合酶链反应的模板。四个RFLP克隆被分配到细胞学定义的5号染色体片段。这与连锁群5的大约三分之一的图谱长度与5号染色体的大约五分之一的有丝分裂中期长度有关。该技术可能有助于将基于RFLP的遗传连锁图谱与大麦染色体的细胞学标记联系起来。
A new strategy has been devised and used for the physical localization of genetically mapped restriction fragment length polymorphism (RFLP) clones to barley chromosomes. Morphologically distinct translocation chromosomes from synchronized root-tip meristems were microisolated and their DNA was used as a template for polymerase chain reaction with sequence-specific primers. Four RFLP clones were assigned to cytologically defined segments of chromosome 5. This related approximately one-third of the map length of linkage group 5 to approximately one-fifth of the mitotic metaphase length of chromosome 5. The technique may substantially contribute to the connection of the RFLP-based genetic linkage maps with cytological markers of the barley chromosomes.