Structural optimization for heat detection of DNA thermosequencing platform using finite element analysis
Structural optimization for heat detection of DNA thermosequencing platform using finite element analysis
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DOI：
10.1063/1.2901138
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发表时间：
2008-04-01
期刊：
影响因子：
3.2
通讯作者：
Davis, Ronald W.
中科院分区：
文献类型：
--
作者：
Esfandyarpour, Hesaam;Zheng, Bo;Davis, Ronald W.
在过去的三十年里，桑格的方法一直是主要的DNA测序技术;然而，成本和复杂性的固有限制限制了其在个性化医学和生态学研究中的应用。一种称为“热测序”的新技术可以通过使用微流体平台潜在地降低DNA测序的成本和复杂性[Esfandyarpour，Pease和Davis，J. Vac. Sci. B26，661（2008）]。为了优化该技术的效率，使用有限元分析通过模拟DNA掺入反应系列和所得产物浓度和产热来对热测序系统进行建模。创建了热测序平台的不同模型，以模拟系统周围材料的影响，优化系统的几何形状，并将反应热集中到特定区域，以便在真实的系统中进行检测。反应产物的所得浓度用于校准反应速度并设计热测序技术中的热传感器。我们推荐使用控制阀对微流体检测平台进行修改的门控结构，并展示了这种新平台如何显着提高检测效率。(c)2008年美国物理学会。
For the past three decades, Sanger's method has been the primary DNA sequencing technology; however, inherent limitations in cost and complexity have limited its usage in personalized medicine and ecological studies. A new technology called "thermosequencing" can potentially reduce both the cost and complexity of DNA sequencing by using a microfluidic platform [Esfandyarpour, Pease, and Davis, J. Vac. Sci. Technol. B26, 661 (2008)]. To optimize the efficiency of the technology, finite element analysis was used to model the thermosequencing system by simulating the DNA incorporation reaction series and the resulting product concentration and heat production. Different models of the thermosequencing platform were created to simulate the effects of the materials surrounding the system, to optimize the geometry of the system, and to concentrate reaction heat into specific regions for detection in the real system. The resulting concentrations of reaction products were used to calibrate the reaction speed and to design the heat sensors in the thermosequencing technology. We recommend a modified gated structure for the microfluidic detection platform by using control valves and show how this new platform could dramatically improve the detection efficiency. (c) 2008 American Institute of Physics.