Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method.
Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method.
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DOI：
10.1155/2016/3961286
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发表时间：
2016
影响因子：
--
通讯作者：
Hayakawa T
中科院分区：
文献类型：
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作者：
Yoshida E;Hayakawa T
据推测，口腔中生物膜的形成导致一些口腔疾病。乳铁蛋白是唾液中的一种抗菌蛋白，是生物被膜形成的重要防御因子。我们分析了吸附量的乳铁蛋白和解离常数（Kd）的乳铁蛋白的不同牙科材料的表面使用平衡分析技术在27 MHz的石英晶体微天平（QCM）测量。评价了四种不同的材料，钛（Ti）、不锈钢（SUS）、氧化锆（ZrO 2）和聚甲基丙烯酸甲酯（PMMA）。将这些材料涂覆到QCM传感器上，并通过原子力显微镜观察、测量表面粗糙度、水接触角和zeta电位来表征表面。QCM测量结果显示，Ti和SUS显示出比ZrO 2和PMMA更大量的乳铁蛋白吸附。表面粗糙度和zeta电位影响乳铁蛋白的吸附。相反，Kd值分析表明，吸附的乳铁蛋白绑定不太紧密的Ti和SUS表面比ZrO2和PMMA表面。乳铁蛋白与ZrO 2和PMMA之间的疏水相互作用被认为参与了乳铁蛋白与ZrO 2和PMMA表面的更好结合。结果表明，乳铁蛋白的吸附行为受到材料表面特性的影响。
It is postulated that biofilm formation in the oral cavity causes some oral diseases. Lactoferrin is an antibacterial protein in saliva and an important defense factor against biofilm development. We analyzed the adsorbed amount of lactoferrin and the dissociation constant (K d) of lactoferrin to the surface of different dental materials using an equilibrium analysis technique in a 27 MHz quartz crystal microbalance (QCM) measurement. Four different materials, titanium (Ti), stainless steel (SUS), zirconia (ZrO2) and polymethyl methacrylate (PMMA), were evaluated. These materials were coated onto QCM sensors and the surfaces characterized by atomic force microscopic observation, measurements of surface roughness, contact angles of water, and zeta potential. QCM measurements revealed that Ti and SUS showed a greater amount of lactoferrin adsorption than ZrO2 and PMMA. Surface roughness and zeta potential influenced the lactoferrin adsorption. On the contrary, the K d value analysis indicated that the adsorbed lactoferrin bound less tightly to the Ti and SUS surfaces than to the ZrO2 and PMMA surfaces. The hydrophobic interaction between lactoferrin and ZrO2 and PMMA is presumed to participate in better binding of lactoferrin to ZrO2 and PMMA surfaces. It was revealed that lactoferrin adsorption behavior was influenced by the characteristics of the material surface.