Correlation of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, and clinical features of hematological disorders:: a pilot study
Correlation of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, and clinical features of hematological disorders:: a pilot study
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DOI：
10.1016/s0145-2126(00)00006-0
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发表时间：
2000-06-01
影响因子：
2.7
通讯作者：
Kamihira, S
中科院分区：
文献类型：
--
作者：
Honda, M;Yamada, Y;Kamihira, S
8-羟基-2'-脱氧鸟苷 (8-OHdG) 是目前最流行的 DNA 氧化损伤标记物，据报道，其水平在各种恶性肿瘤患者中升高。在本研究中，通过酶联免疫吸附测定对 44 名血液病患者（13 名恶性淋巴瘤、11 名成人 T 细胞白血病/淋巴瘤 (ATL)、10 名急性白血病和 10 名骨髓增生异常综合征 (MDS)）的尿 8-OHdG 进行了检查。与正常对照相比，ATL患者治疗前尿8-OHdG水平显着升高（25.3±12.9 vs. 11.9±7.3ng/mg，P<0.05）。尽管淋巴瘤、急性白血病和MDS患者的尿8-OHdG水平也高于正常对照，但差异并不显着。然而，两名患有转化中原始细胞过多的难治性贫血 (RAEB-t) 且具有极端单核细胞增多和中性粒细胞增多的患者表现出异常高的尿 8-OHdG 水平（161.0 和 218.9 ng/mg）。化疗后尿 8-OHdG 排泄量短暂增加，无论疾病如何，这种波动都很显着（P < 0.05）。有趣的是，高LDH、晚期、体能状态差或国际预后指数（IPI）高/高中危的淋巴瘤患者尿8-OHdG水平显着升高（P < 0.05- < 0.001）。后面的这些结果表明尿 8-OHdG 可能是淋巴瘤患者可靠的预后标志物，并且应该鼓励大规模和长期的随访研究。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
The 8-hydroxy-2'-deoxyguanosine (8-OHdG), presently the most popular marker for oxidative DNA damage, level has been reported to be elevated in patients with various malignancies. In the present study, urinary 8-OHdG was examined in 44 patients with hematological disorders (13 malignant lymphoma, 11 adult T cell leukemia/lymphoma (ATL), 10 acute leukemia, and 10 myelodysplastic syndrome (MDS)) by an enzyme-linked immunosorbent assay. The pre-therapy level of urinary 8-OHdG in ATL patients was significantly elevated compared with normal controls (25.3 +/- 12.9 vs. 11.9 +/- 7.3 ng/mg, P < 0.05). Although patients with lymphoma, acute leukemia and MDS also showed higher urinary 8-OHdG levels than normal controls, the differences were not significant. However, two patients with refractory anemia with excess blasts in transformation (RAEB-t) having extreme monocytosis and neutrophilia showed exceptionally high urinary 8-OHdG levels (161.0 and 218.9 ng/mg). Urinary 8-OHdG excretion increased transiently with chemotherapy, and this fluctuation was significant irrespective of the disorder (P < 0.05). Interestingly, lymphoma patients with high LDH, advanced stage, poor performance status or International Prognostic Index (IPI) of high/high-intermediate risk had significantly elevated urinary 8-OHdG levels (P < 0.05- < 0.001). These latter results suggest that urinary 8-OHdG may be a reliable prognostic marker in lymphoma patients and should encourage large scale and long term follow up studies. (C) 2000 Elsevier Science Ltd. All rights reserved.