A morphologically distinct granule cell type in the dentate gyrus of the red fox correlates with adult hippocampal neurogenesis
A morphologically distinct granule cell type in the dentate gyrus of the red fox correlates with adult hippocampal neurogenesis
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DOI：
10.1016/j.brainres.2010.02.075
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发表时间：
2010-04-30
期刊：
影响因子：
2.9
通讯作者：
Slomianka, Lutz
中科院分区：
文献类型：
--
作者：
Amrein, Irmgard;Slomianka, Lutz
野生红狐是公认的狡猾的食肉动物，对成年海马神经发生和齿状回的形态特征进行了研究。成年红狐的齿状回中年轻的双皮质醇阳性神经元几乎是驯养狗的15倍。双重皮质素阳性细胞占颗粒细胞总数的4.4%，分裂细胞仅占0.06%。与实验室小鼠相比，增殖(Ki67阳性)和死亡的细胞很少，但新神经元的比例非常相似。增殖细胞、神经细胞系的年轻细胞和死亡细胞的数量相关。常驻颗粒细胞可分为两种类型，它们的形态、染色模式和明显的时空分布截然不同。细胞核直径为7.3微米的小颗粒细胞占所有颗粒细胞的83%。剩下的颗粒细胞明显较大，核直径为9.4微米，NeuN染色较深。7月和中期，密集排列的小细胞占主导地位。在这里，只有少数大颗粒细胞散布在整个细胞层。在时间上，颗粒细胞变得更加松散，大多数细胞是大的类型。在大颗粒细胞数量较多的狐狸中观察到较高的神经形成率，而小颗粒细胞的数量与任何与神经发生相关的细胞计数都不相关。小白蛋白、谷氨酸受体2/3、GAP-43和强啡肽的染色显示了一个解剖背景，这是其他哺乳动物物种共同的特征的组合。综上所述，我们报道了一种形态上不同的颗粒细胞类型，它与成年狐狸的海马神经发生有关。此外，像其他长寿物种如灵长类动物一样，年轻神经元的成熟期可能会延长。(C)2010爱思唯尔B.V.保留所有权利。
Wild red foxes, proverbially cunning carnivores, are investigated for adult hippocampal neurogenesis and morphological characteristics of the dentate gyrus. Adult red foxes harbor almost 15-times more young, doublecortin-positive neurons in their dentate gyrus than domesticated dogs. The number of doublecortin-positive cells corresponds to 4.4% of the total granule cell number, whereas dividing cells amount to only 0.06%. Compared to laboratory mice, proliferating (Ki67-positive) and dying cells are rare, but the percentage of new neurons is quite similar. The numbers of proliferating cells, young cells of neuronal lineage and dying cells correlate. Resident granule cells can be divided into two types with strikingly different morphologies, staining patterns and distinct septotemporal distributions. Small sized granule cells with a nuclear diameter of 7.3 mu m account for similar to 83% of all granule cells. The remaining granule cells are significantly larger with a nuclear diameter of 9.4 mu m diameter and stain heavily for NeuN. Septally and mid-septotemporally, densely packed small cells dominate. Here, only few large granule cells are scattered throughout the layer. Temporally, granule cells become more loosely packed and most of the cells are of the large type. High rates of neurogenesis are observed in foxes with high numbers of large granule cells, whereas the number of small granule cells does not correlate with any of the neurogenesis-related cell counts. Staining for parvalbumin, glutamate receptor 2/3, GAP-43 and dynorphin shows an anatomical context that is a composite of features common also to other mammalian species. In summary, we report a morphologically distinct granule cell type which correlates with adult hippocampal neurogenesis in the fox. Furthermore, the maturation phase of the young neurons may be prolonged as in other long living species such as primates. (C) 2010 Elsevier B.V. All rights reserved.