UV Light Killing Efficacy of Fluorescent Protein-Expressing Cancer Cells In Vitro and In Vivo
UV Light Killing Efficacy of Fluorescent Protein-Expressing Cancer Cells In Vitro and In Vivo
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DOI：
10.1002/jcb.22693
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发表时间：
2010-08-15
影响因子：
4
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Kimura, Hiroaki;Lee, Claudia;Hoffman, Robert M.
我们研究了UV光对在细胞核中表达GFP和在细胞质中表达RFP的癌细胞（双色细胞）的细胞杀伤效力。暴露于不同剂量的UVA、UVB或UVC后，在荧光显微镜下使用双色143 B人骨肉瘤细胞、HT-1080人纤维肉瘤细胞、刘易斯肺癌（LLC）和XPA-1人胰腺癌细胞体外定量凋亡和活细胞。紫外线诱导的癌细胞死亡是波长和剂量依赖性的，以及细胞系依赖性。在OVA暴露后，即使UV剂量增加到200 J/m（2），大多数细胞仍能存活。用UVB照射，在50 J/m2的照射下观察到细胞死亡。对于UVC，低至25 J/m2的UVC照射杀死了约70%的143 B双色细胞。这种剂量的UVB或UVA对癌细胞几乎没有影响。UV诱导的癌细胞死亡在细胞系之间变化。照射后4 h细胞开始死亡，并持续至照射后10 h。UVC暴露也抑制了裸鼠微小残留癌（MRC）模型中癌细胞的生长。没有观察到UVC暴露的明显副作用。这项研究开辟了手术切除后MRC的UVC治疗的可能性。J.细胞。110：1439-1446，2010. (C)2010 Wiley-Liss，Inc.
We investigated the cell-killing efficacy of UV light on cancer cells expressing GFP in the nucleus and RFP in the cytoplasm (dual-color cells). After exposure to various doses of UVA, UVB, or UVC, apoptotic and viable cells were quantitated under fluorescence microscopy using dual-color 143B human osteosarcoma cells, HT-1080 human fibrosarcoma cells, Lewis lung carcinoma (LLC), and XPA-1 human pancreatic cancer cells in vitro. UV-induced cancer cell death was wave-length and dose dependent, as well as cell-line dependent. After OVA exposure, most cells were viable even when the UV dose was increased up to 200 J/m(2). With UVB irradiation, cell death was observed with irradiation at 50 J/m(2). For UVC, as little as 25 J/m(2) UVC irradiation killed approximately 70% of the 143B dual-color cells. This dose of UVB or UVA had almost no effect on the cancer cells. UV-induced cancer cell death varied among the cell lines. Cell death began about 4 h after irradiation and continued until 10 h after irradiation. UVC exposure also suppressed cancer cell growth in nude mice in a model of minimal residual cancer (MRC). No apparent side effects of UVC exposure were observed. This study opens up the possibility of UVC treatment for MRC after surgical resection. J. Cell. Biochem. 110: 1439-1446, 2010. (C) 2010 Wiley-Liss, Inc.