Selective Nanopatterning Using Citrate-Stabilized Au Nanoparticles and Cystein-Modified Amphiphilic Protein
Selective Nanopatterning Using Citrate-Stabilized Au Nanoparticles and Cystein-Modified Amphiphilic Protein
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DOI：
10.1021/la804001e
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发表时间：
2009-05-05
期刊：
影响因子：
3.9
通讯作者：
Linder, Markus B.
中科院分区：
文献类型：
--
作者：
Laaksonen, Paivi;Kivioja, Jani;Linder, Markus B.
我们提出了一种方法，其中生物分子自组装与光刻技术相结合，在硅衬底上产生金属纳米颗粒图案。这是通过两步法实现的，结果是将纳米颗粒附着在样品表面的所需位置上，从而允许进行详细的表征。首先，基因修饰的疏水蛋白NCysHFBI通过自组装附着在疏水表面或带有疏水和亲水结构域的表面上。下一步是用17.8纳米金纳米颗粒标记蛋白质层，以便对薄膜进行微观表征。用紫外-可见光谱和透射电子显微镜表征了纳米粒子的附着动力学和附着程度。结果表明，柠檬酸稳定的金纳米粒子的附着强烈依赖于封端配位层的静电性质，单分子层中纳米粒子的密度可以通过pH来控制。得到的纳米颗粒组件遵循高精度的光学光刻产生的原始图案。我们证明，在良好的平衡中结合自下而上和自上而下的纳米技术方法，可以提供非常有效的方法来生产纳米级组件，提供电子设备和生物世界之间的功能接口。
We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV-vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.