A 31P-NMR study of some metabolic and functional effects of the inotropic agents epinephrine and ouabain, and the ionophore R02-2985 (X537A) in the isolated, perfused rat heart.
A 31P-NMR study of some metabolic and functional effects of the inotropic agents epinephrine and ouabain, and the ionophore R02-2985 (X537A) in the isolated, perfused rat heart.
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31P-NMR 研究正性肌力药物肾上腺素和哇巴因以及离子载体 R02-2985 (X537A) 在离体灌注大鼠心脏中的一些代谢和功能影响。
DOI：
10.1016/0167-4889(82)90008-8
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Radda,GK
中科院分区：
文献类型：
--
作者：
Matthews,PM;Williams,SR;Seymour,AM;Schwartz,A;Dube,G;Gadian,DG;Radda,GK
用31P-核磁共振仪表征了朗宁多夫灌流的大鼠心脏机械活动增加的某些代谢效应。输注哇巴因(0.9−7.0.10−5M)、离子载体R02-2985(1·10−5M)或肾上腺素(5·10−8M)可增加机械活动。2.以含11 mM葡萄糖为底物的缓冲液灌流心脏时，每种正性肌力药的代谢变化相似。在每种情况下，磷酸肌酸浓度都有所下降。在肾上腺素输注期间，磷酸肌酸开始恢复其原始浓度，尽管机械活动没有明显变化。3.比较了葡萄糖(11 MM)、醋酸盐(5 MM)和乳酸盐(5 MM)对肾上腺素正性变力和变时性作用心脏代谢的影响。在以乳酸为唯一外源性底物的心脏中注入肾上腺素时，磷酸肌酸浓度也呈时间依赖性下降，但以乙酸盐为底物的相同肾上腺素注入后，没有观察到有统计学意义的代谢物变化。4.在肌酸磷酸激酶反应中假定平衡的游离ADP浓度的计算允许估计胞浆磷酸盐势([ATP][ADP][PI])，这似乎取决于许多因素，包括外源底物的性质和机械活动的水平。5.因此，我们得出结论：在大鼠心脏灌流过程中，磷酸电位与线粒体呼吸频率之间没有普遍的相关性。
Some metabolic effects of increased mechanical activity by the Langendorff-perfused rat heart have been characterized using 31 P-NMR. Mechanical activity was increased by infusion of ouabain (0.9− 7.0· 10− 5 M), the ionophore R02-2985 (1· 10− 5 M) or epinephrine (5· 10− 8 M). 2. Similar metabolic changes accompanied infusion of each of the positive inotropic agents into hearts perfused with buffer containing 11 mM glucose as the substrate. In each case phosphocreatine concentrations decreased. During the period of epinephrine infusion the phosphocreatine began to recover its original concentration, although there were no significant changes in mechanical activity. 3. Comparisons of the metabolic changes accompanying the positive inotropic and chronotropic effects of epinephrine were made between hearts perfused with either glucose (11 mM), acetate (5 mM) or lactate (5 mM). A time-dependent decrease in phosphocreatine concentrations also accompanied infusion of epinephrine into hearts perfused with lactate as the sole exogenous substrate, but no statistically significant metabolite changes were observed after identical epinephrine infusions with acetate as the substrate. 4. Calculation of the concentration of free ADP assuming equilibrium in the creatine phosphokinase reaction allows estimation of the cytosolic phosphate potential ([ATP][ADP][P i]), which appears to be dependent on a number of factors, including the nature of the exogenous substrate and the level of mechanical activity. 5. Thus, we conclude that there is no general correlation between the phosphate potential and the mitochondrial respiratory rate in the perfused rat heart.
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影响因子：
20.1
作者：
KOBAYASHI, K;NEELY, JR
通讯作者：
NEELY, JR
心脏做功增加对三羧酸循环的影响及其与灌注大鼠心脏中糖酵解的相互作用。
影响因子：
4.1
作者：
J. R. Neely;R. Denton;P. England;P. J. Randle
通讯作者：
P. J. Randle
新型正性肌力药物的血流动力学和生化作用。
影响因子：
20.1
作者：
A. Schwartz;R. Lewis;H. Hanley;R. Munson;Frederick D. Dial;M. V. Ray
通讯作者：
M. V. Ray
胞质磷酸化电位。
影响因子：
4.8
作者：
R. Veech;J. Lawson;N. Cornell;H. Krebs
通讯作者：
H. Krebs
离体灌注大鼠心脏磷酸化电位与耗氧量的关系。
影响因子：
5
作者：
J. Giesen;H. Kammermeier
通讯作者：
H. Kammermeier