Astrocytic factors down-regulate the expression of major histocompatibility complex-class-II and intercellular adhesion molecule-1 on human monocytes
Astrocytic factors down-regulate the expression of major histocompatibility complex-class-II and intercellular adhesion molecule-1 on human monocytes
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DOI：
10.1016/s0304-3940(00)01711-0
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发表时间：
2001-01-26
影响因子：
2.5
通讯作者：
Blaheta, RA
中科院分区：
文献类型：
--
作者：
Hailer, NP;Glomsda, B;Blaheta, RA
几个因素有助于维持中枢神经系统的免疫赦免和星形胶质细胞已被确定为免疫调节细胞因子的主要来源。为了研究造血单核细胞是否被星形胶质细胞衍生因子免疫失活，用脂多糖或干扰素（IFN）-γ刺激人单核细胞，并用来自纯星形胶质细胞培养物的上清液、白细胞介素（IL）-4、IL-10或IL-1受体拮抗剂（IL-1-RA）处理人单核细胞。流式细胞术表明，从星形胶质细胞培养物的上清液是最有效的代理在减少主要组织相容性复合物（MHC）-II类-以及细胞间粘附分子-1-表达的水平，而IL-4，IL-10，IL-1-RA只有边际效应。白细胞功能抗原-1和极晚期抗原-4的表达不受任何因素的调节。总之，星形胶质细胞似乎提供了可溶性因子，有能力使造血单核细胞失活。(C)2001爱思唯尔科学爱尔兰有限公司版权所有。
Several factors contribute to the maintenance of central nervous system immune privilege and astrocytes have been identified as a major source of immunomodulatory cytokines. To investigate whether hematogenous monocytes are immunologically deactivated by astrocyte-derived factors human monocytes were stimulated with lipopolysaccharide or interferon (IFN)-gamma and treated with the supernatant from pure astrocyte cultures, interleukin (IL)-4, IL-10, or with IL-1-receptor antagonist (1L-1-RA). Flow cytometry demonstrated that the supernatant from astrocyte cultures was the most potent agent in reducing the levels of major histocompatibility complex (MHC)-class-II- as well as intercellular adhesion molecule-1-expression, whereas IL-4, IL-10, and IL-1-RA had only marginal effects. The expression of leukocyte function antigen-1 and very late antigen-4 was not modulated by either factor. In conclusion, astrocytes seem to provide soluble factors that have the capacity to deactivate hematogenous monocytes. (C) 2001 Elsevier Science ireland Ltd. All rights reserved.