Chlamydial serology: Comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens
Chlamydial serology: Comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens
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DOI：
10.1128/jcm.39.4.1368-1377.2001
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发表时间：
2001-04-01
影响因子：
9.4
通讯作者：
Vischer, TL
中科院分区：
文献类型：
--
作者：
Bas, S;Muzzin, P;Vischer, TL
为了提高沙眼衣原体感染血清学诊断的可靠性，采用免疫印迹分析、微量免疫荧光滴定和不同的免疫测定法，使用主要外膜蛋白可变区IV中的种特异性表位或重组抗原的合成肽衍生（热休克蛋白70 [hsp 70]、hsp 60、hsp 10、由质粒[pgp 3]的开放阅读框3编码的多肽、巨噬细胞感染性增强剂和总脂多糖的片段）进行评价，由于衣原体种属之间的交叉反应已有报道，因此还使用肺炎衣原体和鹦鹉热衣原体作为抗原进行了微量免疫荧光试验，并通过免疫测定法测定了肺炎衣原体特异性抗体。由于抗菌抗体的存在必须根据其在一般人群中的流行率进行解释，用来自明确感染的患者的血清样品获得的应答（即，尿道或宫颈内沙眼衣原体DNA扩增阳性）与健康献血员标本进行比较。免疫印迹结果的最佳敏感性（86%）和特异性为81%，当个体数对不同的C、IgM抗体反应大于或等于10和/或大于或等于2时，考虑沙眼抗原。大多数样品识别13-kDa抗原在急性泌尿生殖道感染患者中，IgG阳性率为86%，但在健康献血员中很少（3%）（P < 0.0001），血清抗体对多肽或重组抗原的敏感性和特异性结果略低于对沙眼衣原体全抗原的应答数，当考虑对重组hsp 60和pgp 3的IgG应答时，分别为76%和77%。
To improve the reliability of the serodiagnosis of Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived front species-specific epitopes in variable domain IV of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipopolysaccharide) were evaluated, Because cross-reactions between chlamydial species have been reported, the microimmunofluorescence tests were also performed with Chlamydia pneumoniae and Chlamydia psittaci used as antigens, and C, pneumoniae-specific antibodies were also determined by immunoassays, Since the presence of antimicrobial antibodies must be interpreted in light of their prevalence in the general population, responses obtained with serum samples from patients with well-defined infection (i.e., with positive urethral or endocervical C, trachomatis DNA amplification) were compared to those obtained with samples from healthy blood donors, The best sensitivity (86%) with a specificity of 81% was obtained for immunoblotting results, when the number of individuals with greater than or equal to 10 immunoglobulin G (Igc) and/or greater than or equal to2 IgM responses to the different C, trachomatis antigens was considered. A 13-kDa antigen was recognized by most of the samples (86% for IgG) front patients with acute urogenital infection but rarely (3%) by those from healthy blood donors (P < 0.0001), The sensitivity and specificity results obtained for serum antibodies to peptides or recombinant antigens were slightly lower than those results obtained for the number of responses to whole C, trachomatis antigens, which were 76 and 77%, respectively, when IgG responses to both recombinant hsp60 and pgp3 were considered.