Characterization of insulin-like growth factor binding proteins of cultured rat astroglial and neuronal cells.
Characterization of insulin-like growth factor binding proteins of cultured rat astroglial and neuronal cells.
复制标题
培养的大鼠星形胶质细胞和神经元细胞的胰岛素样生长因子结合蛋白的表征。
DOI：
10.1016/0006-291x(89)92254-7
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Rosenfeld,RG
中科院分区：
文献类型：
--
作者：
Ocrant,I;Pham,H;Oh,Y;Rosenfeld,RG
胰岛素样生长因子结合蛋白产生的培养大鼠神经元，星形胶质细胞，和大鼠细胞系BRL-3A和B104进行了比较，结合蛋白在大鼠血清中发现，使用亲和标记，去糖基化，和Western配体印迹研究。每个来源阐述了一个独特的模式的异质性结合蛋白。来自不同来源的一些结合蛋白在每个实验系统中表现相似，表明这些结合蛋白的子集可能在结构上相关。特别是，我们的数据表明，培养的星形胶质细胞和神经元，使BRL-3A细胞产生的主要结合蛋白。
Insulin-like growth factor binding proteins produced by cultured rat neurons, astrocytes, and rat cell lines BRL-3A and B104 were compared to binding proteins found in rat serum, using affinity labeling, deglycosylation, and Western ligand blotting studies. Each source elaborated an unique pattern of heterogeneous binding proteins. Some of the binding proteins from different sources behaved similarly in each experimental system suggesting that subsets of these binding proteins may be structurally related. In particular, our data suggest that cultured astrocytes and neurons make the major binding protein produced by BRL-3A cells.