Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.
Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.
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DOI：
10.1038/nbt.3071
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发表时间：
2015-01
影响因子：
46.9
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
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作者：
Kim, Jaeyun;Li, Weiwei Aileen;Choi, Youngjin;Lewin, Sarah A.;Verbeke, Catia S.;Dranoff, Glenn;Mooney, David J.
植入体内对宿主免疫细胞进行编程的材料是一种有希望的替代方案，可以替代移植体外操纵的细胞来指导免疫反应，但需要进行外科手术。在这里，我们证明了高纵横比的介孔硅棒(MSR)注射针会在体内自发组装形成大孔结构，为宿主免疫细胞提供3D细胞微环境。在小鼠体内，大量的DC被招募到支架杆之间的毛孔中。树突状细胞的募集和随后的归巢可以通过从支架上持续释放炎症信号和佐剂来调节。此外，与推注对照相比，注射基于MSR的疫苗配方可提高全身TH1和TH2血清抗体和细胞毒T细胞水平。这些发现表明，可注射MSR可能作为一种多功能疫苗平台来调节宿主免疫细胞功能并激发适应性免疫反应。
Materials implanted in the body to program host immune cells are a promising alternative to transplantation of ex vivo–manipulated cells to direct an immune response, but required a surgical procedure. Here we demonstrate that high-aspectratio, mesoporous silica rods (MSRs) injected with a needle spontaneously assemble in vivo to form macroporous structures that provide a 3D cellular microenvironment for host immune cells. In mice, substantial numbers of DCs are recruited to the pores between the scaffold rods. The recruitment of DCs and their subsequent homing to lymph nodes can be modulated by sustained release of inflammatory signals and adjuvants from the scaffold. Moreover, injection of an MSR-based vaccine formulation enhances systemic TH1 and TH2 serum antibody and cytotoxic T cell levels compared to bolus controls. These findings suggest that injectable MSRs may serve as a multifunctional vaccine platform to modulate host immune cell function and provoke adaptive immune responses.
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影响因子：
--
作者：
Jähnisch H;Füssel S;Kiessling A;Wehner R;Zastrow S;Bachmann M;Rieber EP;Wirth MP;Schmitz M
通讯作者：
Schmitz M
影响因子：
41.2
作者：
通讯作者：
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影响因子：
41.2
作者：
通讯作者：
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DOI：
10.1073/pnas.1112648109
发表时间：
2012-01-24
影响因子：
11.1
作者：
Moon, James J.;Suh, Heikyung;Irvine, Darrell J.
通讯作者：
Irvine, Darrell J.
影响因子：
15.3
作者：
Dieu, M C;Vanbervliet, B;Vicari, A;Bridon, J M;Oldham, E;Ait-Yahia, S;Briere, F;Zlotnik, A;Lebecque, S;Caux, C
通讯作者：
Caux, C