Bioactive amorphous magnesium phosphate-polyetheretherketone composite filaments for 3D printing.
Bioactive amorphous magnesium phosphate-polyetheretherketone composite filaments for 3D printing.
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DOI：
10.1016/j.dental.2020.04.008
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
P. Sikder;J. Ferreira;Ehsan Akbari Fakhrabadi;K. Z. Kantorski;M. Liberatore;M. Bottino;S. Bhaduri
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文献类型：
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作者：
P. Sikder;J. Ferreira;Ehsan Akbari Fakhrabadi;K. Z. Kantorski;M. Liberatore;M. Bottino;S. Bhaduri
本研究的目的是开发具有生物活性和骨整合性的聚醚醚酮（PEEK）基复合材料长丝与新型无定形磷酸镁（AMP）颗粒熔融混合，用于牙科和骨科植入物的3D打印。材料和方法对AMP-PEEK进行了一系列材料和生物学分析。测定了AMP的热稳定性、热重曲线和差示扫描量热曲线。用旋转流变仪测定了复合粘度、弹性模量和粘性模量，用模拟体液浸泡法分析了其体外生物活性。利用SEM、EDS和XRD等手段研究了AMP-PEEK纤维的磷灰石形成能力。培养小鼠前成骨细胞（MC 3 T3-E1）并分析细胞活力、增殖和基因表达。在体内分析中，以裸PEEK作为对照，并根据其在体外细胞中的相关结果选择15 AMP-PEEK。4周或12周后，动物被安乐死，并收集股骨进行显微计算机断层扫描（μ-CT）和histologics.ResultsThe收集的结果证实了AMP颗粒在PEEK基质中的均匀分散，无相降解。流变学研究表明，AMP-PEEK复合材料表现出高零剪切粘度和低无限剪切粘度，是3D打印的良好候选材料。体外结果显示，与裸PEEK相比，AMP-PEEK复合材料具有增强的生物活性和上级前成骨细胞功能。体内分析进一步证实了AMP-PEEK植入物增强的骨整合能力。目前的研究表明，AMP-PEEK复合材料长丝由于增强的生物活性和骨整合能力，可以用作整形外科和牙科植入物的3D打印的原料。
ObjectiveThe aim of this study was to develop bioactive and osseointegrable polyetheretherketone (PEEK)-based composite filaments melt-blended with novel amorphous magnesium phosphate (AMP) particles for 3D printing of dental and orthopedic implants.Materials and methodsA series of materials and biological analyses of AMP-PEEK were performed. Thermal stability, thermogravimetric and differential scanning calorimetry curves of as-synthesized AMP were measured. Complex viscosity, elastic modulus and viscous modulus were determined using a rotational rheometer.In vitrobioactivity was analyzed using SBF immersion method. SEM, EDS and XRD were used to study the apatite-forming ability of the AMP-PEEK filaments. Mouse pre-osteoblasts (MC3T3-E1) were cultured and analyzed for cell viability, proliferation and gene expression. Forin vivoanalyses, bare PEEK was used as the control and 15AMP-PEEK was chosen based on itsin vitrocell-related results. After 4 or 12 weeks, animals were euthanized, and the femurs were collected for micro-computed tomography (μ-CT) and histology.ResultsThe collected findings confirmed the homogeneous dispersion of AMP particles within the PEEK matrix with no phase degradation. Rheological studies demonstrated that AMP-PEEK composites are good candidates for 3D printing by exhibiting high zero-shear and low infinite-shear viscosities.In vitroresults revealed enhanced bioactivity and superior pre-osteoblast cell function in the case of AMP-PEEK composites as compared to bare PEEK.In vivoanalyses further corroborated the enhanced osseointegration capacity for AMP-PEEK implants.SignificanceCollectively, the present investigation demonstrated that AMP-PEEK composite filaments can serve as feedstock for 3D printing of orthopedic and dental implants due to enhanced bioactivity and osseointegration capacity.