Biotechnological applications of plant freezing associated proteins.
Biotechnological applications of plant freezing associated proteins.
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DOI：
10.1016/s1387-2656(00)06019-1
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Sarhan, F
中科院分区：
文献类型：
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作者：
Breton, G;Danyluk, J;Sarhan, F
植物利用各种蛋白质来保护自己免受低温和冷冻条件的影响。这些抗冻相关蛋白的鉴定及其抗冻功能的阐明将在许多领域具有重要的应用价值。已使用编码结构蛋白、渗压物产生酶、氧化应激清除酶、脂质去饱和酶和基因调节剂的基因来产生转基因植物。这些研究揭示了不同基因的潜在能力，以防止温度相关的压力。在某些情况下，已经产生了具有显著耐冷性的转基因植物。此外，冷诱导的抗冻蛋白和抗冻蛋白的生物化学特性揭示了在食品和医药工业中的许多应用。这些蛋白质被认为是食品添加剂，以提高冷冻食品的质量和保质期，作为冷冻保护剂的器官和细胞冷冻保存，并作为化学佐剂在癌症冷冻手术。
Plants use a wide array of proteins to protect themselves against low temperature and freezing conditions. The identification of these freezing tolerance associated proteins and the elucidation of their cryoprotective functions will have important applications in several fields. Genes encoding structural proteins, osmolyte producing enzymes, oxidative stress scavenging enzymes, lipid desaturases and gene regulators have been used to produce transgenic plants. These studies have revealed the potential capacity of different genes to protect against temperature related stresses. In some cases, transgenic plants with significant cold tolerance have been produced. Furthermore, the biochemical characterization of the cold induced antifreeze proteins and dehydrins reveals many applications in the food and the medical industries. These proteins are being considered as food additives to improve the quality and shelf-life of frozen foods, as cryoprotective agents for organ and cell cryopreservation, and as chemical adjuvant in cancer cryosurgery.