Enterococcus faecalis cytolysin without effect on the intestinal growth of susceptible enterococci in mice.
Enterococcus faecalis cytolysin without effect on the intestinal growth of susceptible enterococci in mice.
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粪肠球菌溶细胞素对小鼠肠道内敏感肠球菌的生长没有影响。
DOI：
10.1093/infdis/172.1.273
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gilmore,MS
中科院分区：
文献类型：
--
作者：
Huycke,MM;Joyce,WA;Gilmore,MS
建立小鼠模型以确定粪肠球菌溶细胞素是否通过其对革兰氏阳性菌的溶菌作用促进溶细胞菌株的肠道过度生长。E.将具有不同溶细胞产物和对溶细胞活性敏感性的粪菌菌株以1：1混合，并允许在肉汤中进行体外竞争或在用抗生素预处理的小鼠中口胃给药后进行体内竞争。在一般情况下，溶细胞菌株超过，多达2000倍，竞争溶细胞素敏感或hypersenseptible菌株在体外。相比之下，在体内没有观察到生长优势，尽管溶细胞和溶细胞素敏感菌株在小鼠肠道的瞬时定植相似。这些数据表明，溶细胞素通过溶菌活性在促进肠球菌的肠道过度生长中起着很小的作用。
A murine model was developed to determine whether theEnterococcus faecaliscytolysin, through its bacteriolytic action on gram-positive bacteria, could promote intestinal overgrowth of cytolytic strains. Sets ofE. faecalisstrains with varying cytolytic production and susceptibility to cytolytic activity were mixed 1:1 and allowed to compete in vitro in broth or in vivo after orogastric administration in mice pretreated with antibiotics. In general, cytolytic strains outgrew, by as much as 2000fold, competing cytolysin-susceptible or -hypersusceptible strains in vitro. In contrast, no growth advantage was observed in vivo, despite similar transient colonization of the murine intestinal tract by both cytolytic and cytolysin-susceptible strains. These data suggest that cytolysin plays little role in promoting intestinal overgrowth of enterococci through bacteriolytic activity.