Effect of Steric Constraint at the γ-Backbone Position on the Conformations and Hybridization Properties of PNAs.
Effect of Steric Constraint at the γ-Backbone Position on the Conformations and Hybridization Properties of PNAs.
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DOI：
10.4061/2011/652702
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发表时间：
2011
影响因子：
2.3
通讯作者：
Ly DH
中科院分区：
文献类型：
--
作者：
Crawford MJ;Rapireddy S;Bahal R;Sacui I;Ly DH
构象上预组织的肽核酸（PNA）已经通过在γ-位置处的骨架修饰来合成，其中R =丙氨酸、缬氨酸、异亮氨酸和苯丙氨酸侧链。使用CD和UV-Vis光谱技术的组合，这些侧链上的构象和杂交性能的PNA的影响进行了测定。我们的研究结果表明，γ-位置可以容纳不同程度的空间位阻侧链，重申了PNA作为“肽”和“核酸”的真正杂交体的双峰功能。
Conformationally preorganized peptide nucleic acids (PNAs) have been synthesized through backbone modifications at the γ-position, where R = alanine, valine, isoleucine, and phenylalanine side chains. The effects of these side-chains on the conformations and hybridization properties of PNAs were determined using a combination of CD and UV-Vis spectroscopic techniques. Our results show that the γ-position can accommodate varying degrees of sterically hindered side-chains, reaffirming the bimodal function of PNAs as the true hybrids of “peptides” and “nucleic acids.”