Retroviral vector-mediated gene transfer into human hematopoietic progenitor cells.
Retroviral vector-mediated gene transfer into human hematopoietic progenitor cells.
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逆转录病毒载体介导的基因转移到人类造血祖细胞中。
DOI：
10.1126/science.3864246
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Jolly,DJ
中科院分区：
文献类型：
--
作者：
Gruber,HE;Finley,KD;Hershberg,RM;Katzman,SS;Laikind,PK;Seegmiller,JE;Friedmann,T;Yee,JK;Jolly,DJ
通过使用逆转录病毒载体将人类次黄嘌呤磷酸核糖转移酶（HPRT）基因转移到人类骨髓细胞中。这些细胞在体外用一种不能复制的鼠逆转录病毒载体感染，该载体携带并表达突变的HPRT互补DNA。受感染的细胞被辅助病毒重复感染并维持在长期培养物中。通过对培养的 HPRT 缺陷、哇巴因抗性鼠成纤维细胞进行集落形成测定，证明了骨髓细胞可产生子代 HPRT 病毒。在集落刺激因子存在下，能够在半固体培养基中形成粒细胞或巨噬细胞（或两者）集落的造血祖细胞存在于非贴壁细胞群中。在琼脂中克隆并随后在液体培养基中培养的集落形成单位产生子代HPRT病毒，表明此类造血祖细胞的感染。
The transfer of the human gene for hypoxanthine phosphoribosyltransferase (HPRT) into human bone marrow cells was accomplished by use of a retroviral vector. The cells were infected in vitro with a replication-incompetent murine retroviral vector that carried and expressed a mutant HPRT complementary DNA. The infected cells were superinfected with a helper virus and maintained in long-term culture. The production of progeny HPRT virus by the bone marrow cells was demonstrated with a colony formation assay on cultured HPRT-deficient, ouabain-resistant murine fibroblasts. Hematopoietic progenitor cells able to form colonies of granulocytes or macrophages (or both) in semisolid medium in the presence of colony stimulating factor were present in the nonadherent cell population. Colony forming units cloned in agar and subsequently cultured in liquid medium produced progeny HPRT virus, indicating infection of this class of hematopoietic progenitor cell.