SPARC is associated with carcinogenesis of oral squamous epithelium and consistent with cell competition.
SPARC is associated with carcinogenesis of oral squamous epithelium and consistent with cell competition.
复制标题
SPARC 与口腔鳞状上皮的癌变有关，并且与细胞竞争一致。
DOI：
10.1007/s00795-014-0089-5
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yamamoto T.
中科院分区：
文献类型：
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作者：
Yamada T;Ohno S;Kitamura N;Sasabe E;Yamamoto T.
基质细胞蛋白是一种酸性分泌蛋白，富含半胱氨酸，被认为与细胞竞争有关。本研究的目的是探讨在口腔鳞状上皮癌变的作用。对57例癌前和早期病变、66例浸润性鳞状细胞癌（SCC）和对照组的临床标本进行了免疫组化染色。对两组患者的临床特征及肿瘤组织学表现进行分析。此外，在口腔癌和角质形成细胞系中检查了cDNA3敲低和过表达的影响。白斑、原位癌和早期浸润性SCC的SPARC阳性细胞数多于正常粘膜上皮。然而，在白斑、原位癌和早期SCC之间没有显著差异，并且在浸润性口腔SCC中，CD14免疫反应性与预后没有相关性。细胞增殖下调的cDNAsiRNA，并增强cDNAs转化角质形成细胞。但是，过表达CD34并不增强细胞迁移活性。癌前病变是由癌变早期的异型增生细胞引起的，可能提高生存能力，但不参与癌变。细胞竞争可能会导致细胞逃避淘汰。
The matricellular protein, secreted protein acidic and rich in cysteine (SPARC) is thought to be involved in cell competition. The objective of this study is to investigate the role of SPARC in cancerization of oral squamous epithelium. Clinical specimens from 57 pre- and early cancerous lesion, 66 invasive squamous cell carcinoma (SCC) and controls were immunostained with SPARC. Clinical features and SPARC expression were evaluated. Furthermore, effects of SPARC knockdown and overexpression were examined in oral cancer and keratinocyte cell lines. Leukoplakia, carcinoma in situ, and early invasive SCC had more SPARC-positive cells than normal mucous epithelium. However, there were no significant differences between leukoplakia, carcinoma in situ, and early SCC, and there were no correlations between SPARC immunoreactivity and prognosis of invasive oral SCCs. Cell proliferation was down-regulated by SPARC siRNA, and enhanced by SPARC transformed keratinocytes. But SPARC overexpression did not enhance cell migration activity. SPARC is induced by dysplastic cells in the early stage of cancerization, and may improve survival capability, but is not involved in malignancy. SPARC may act to escape from elimination by cell competition.