Laser-capture microdissection: opening the microscopic frontier to molecular analysis
Laser-capture microdissection: opening the microscopic frontier to molecular analysis
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DOI：
10.1016/s0168-9525(98)01489-9
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发表时间：
1998-07-01
影响因子：
11.4
通讯作者：
Liotta, LA
中科院分区：
文献类型：
--
作者：
Simone, NL;Bonner, RF;Liotta, LA
随着表达的人类基因列表的扩大，一个主要的科学挑战是了解驱动正常组织形态发生和实际组织病理病变演变的分子事件。激光捕获显微解剖（LCM）的发展是为了提供一种可靠的方法，从组织切片的特定微观区域获得纯细胞群，一步即直接可视化。感兴趣的细胞被转移到由激光脉冲激活的聚合物薄膜上。获得的细胞的确切形态（具有完整的DNA， RNA和蛋白质）被保留并保存在转移膜上。随着LCM的出现，cDNA文库可以从直接从染色组织中获得的纯细胞中开发出来，并且数千个基因的微杂交阵列现在可以用于检查微解剖人体组织活检中的基因表达。与特定疾病阶段相关的表达基因的波动或细胞DNA的改变最终可以在个体患者内部或个体患者之间进行比较。这种基因表达模式的指纹图谱为病因学提供了关键线索，并可能最终有助于诊断决策和针对个体患者的治疗。发现与特定病理病变相关的分子可作为成像或治疗靶点。
As the list of expressed human genes expands, a major scientific challenge is to understand tbe molecular events that drive normal tissue morphogenesis and the evolution of pathological lesions in actual tissue. Laser capture microdissection (LCM) has been developed ro provide a reliable method to procure pure populations of cells from specific microscopic regions of tissue sections, in one step tenner direct visualization. The cells of interest are transferred to a polymer film that is activated by laser pulses. The exact morphology of the procured cells (with intact DNA, RNA and proteins) is retained and held on the transfer film. With the advent of LCM, cDNA libraries can be developed from pure cells obtained directly from stained tissue, and microhybridization arrays of thousands of genes can now be used to examine gene expression in microdissected human tissue biopsies. The fluctuation of expressed genes or alterations in the cellular DNA that correlate with a particular disease stage can ultimately be compared within or between individual patients. Such a fingerprint of gene-expression patterns call provide crucial clues for etiology and might, ultimately, contribute to diagnostic decisions and therapies tailored to the individual patient. Molecules found to be associated with a defined pathological lesion might serve as imaging or therapeutic targets.