Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation
Rapid fabrication of poly(dimethylsiloxane)-based microchip capillary electrophoresis devices using CO2 laser ablation
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DOI：
10.1039/b418299e
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发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
Lunte, SM
中科院分区：
文献类型：
--
作者：
Fogarty, BA;Heppert, KE;Lunte, SM
介绍了用CO2激光烧蚀法在聚二甲基硅氧烷（PDMS）中制备毛细管电泳（CE）微通道的方法。低成本聚合物器件是使用相对便宜的CO2激光系统生产的，该系统促进了微通道的快速图像化和烧蚀。设备设计是使用商业上可用的软件包创建的。研究了PDMS厚度、激光聚焦、功率和速度对通道尺寸的影响。通过优化设置，可以生产的最小通道平均深度为33 mm (11.1% RSD, N = 6)，宽度为110 mm （5.7% RSD, N = 6）。使用PDMS基板可以在室温下对微芯片组件进行可逆密封，而不需要洁净室设施。使用一层预固化的聚合物，设备在几分钟内被设计、烧蚀和组装。最终装置用于微芯片CE分离和荧光标记神经递质天冬氨酸和谷氨酸的检测。
The use of CO2 laser ablation for the patterning of capillary electrophoresis (CE) microchannels in poly( dimethylsiloxane) ( PDMS) is described. Low-cost polymer devices were produced using a relatively inexpensive CO2 laser system that facilitated rapid patterning and ablation of microchannels. Device designs were created using a commercially available software package. The effects of PDMS thickness, laser focusing, power, and speed on the resulting channel dimensions were investigated. Using optimized settings, the smallest channels that could be produced averaged 33 mm in depth ( 11.1% RSD, N = 6) and 110 mm in width (5.7% RSD, N = 6). The use of a PDMS substrate allowed reversible sealing of microchip components at room temperature without the need for cleanroom facilities. Using a layer of pre-cured polymer, devices were designed, ablated, and assembled within minutes. The final devices were used for microchip CE separation and detection of the fluorescently labeled neurotransmitters aspartate and glutamate.