ZEBRAFISH PAX[ZF-A] - A PAIRED BOX-CONTAINING GENE EXPRESSED IN THE NEURAL-TUBE
ZEBRAFISH PAX[ZF-A] - A PAIRED BOX-CONTAINING GENE EXPRESSED IN THE NEURAL-TUBE
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DOI：
10.1002/j.1460-2075.1991.tb04927.x
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发表时间：
1991-12-01
期刊：
影响因子：
11.4
通讯作者：
FJOSE, A
中科院分区：
文献类型：
--
作者：
KRAUSS, S;JOHANSEN, T;FJOSE, A
与果蝇分割基因配对的配对盒同源的鼠类和人类序列先前已被报道。在这里，我们描述了一个斑马鱼（Brachydanio rerio）配对盒包含克隆，pax[zf-a]，这是明显不同于报告的脊椎动物Pax基因。由pax[zf-a]编码的推定蛋白质包含由富含甘氨酸的酸性接头和显著富含丝氨酸、苏氨酸和脯氨酸残基的羧基末端分开的配对盒和配对型同源盒。通过对胚胎组织切片和整个胚胎进行原位杂交，在中枢神经系统和胚胎的限制区域内发现了par[zf-a]转录本。与最近表征的鼠Pax基因相反，pax[zf-a]在分节的中胚层中不表达。在17小时阶段，pax[zf-a]的表达被检测到在间脑的一个确定的区域，该区域限定了假定的丘脑。这表明parzf-al参与了喙部脑中的模式形成。pax[zf-al]基因也在整个后脑和脊髓中表达。该杂交信号被限制在包括基板的纵向柱上。在发育后期，受精后36小时，pax[zfa]转录本不再局限于间脑的特定区域，而是分布在整个发育中的大脑中。
Murine and human sequences homologous to the paired box of the Drosophila segmentation gene paired have been reported previously. Here we describe a zebrafish (Brachydanio rerio) paired box-containing clone, pax[zf-a], which is clearly distinct from reported vertebrate Pax genes. The putative protein encoded by pax[zf-a] contains a paired box and a paired-type homeobox separated by a glycine-rich, acidic linker and a carboxy-terminal end which is remarkably rich in serine, threonine and proline residues. By in situ hybridization to embryonic tissue sections and whole mount embryos, par[zf-a] transcripts were found within restricted regions of the central nervous system and the eve. In contrast to the murine Pax genes recently characterized, pax[zf-a] is not expressed in the segmented mesoderm. At the 17 h stage, pax[zf-a] expression is detected in a defined area of the diencephalon which circumscribes the presumptive thalamus. This suggests an involvement of par[zf-al in pattern formation in the rostral brain. The pax[zf-al gene is also expressed throughout the hindbrain and spinal cord. This hybridization signal is restricted to a longitudinal column which includes the basal plate. Later in development, at 36 h post-fertilization, pax[zfa] transcripts are no longer restricted to a specific region of the diencephalon, but are distributed over the entire developing brain.