PvRON2, a new Plasmodium vivax rhoptry neck antigen.
PvRON2, a new Plasmodium vivax rhoptry neck antigen.
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DOI：
10.1186/1475-2875-10-60
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发表时间：
2011-03-14
期刊：
影响因子：
3
通讯作者：
Patarroyo MA
中科院分区：
文献类型：
--
作者：
Arévalo-Pinzón G;Curtidor H;Patiño LC;Patarroyo MA
棒状体是来自属于顶复门的寄生虫的特化细胞器;它们在侵入宿主靶细胞期间分泌其蛋白质内容物，并在棒状体颈部或球内被分选成离散的亚室。这种分布与这些蛋白质分别在紧密连接（TJ）和寄生虫空泡（PV）形成中的作用有关。以恶性疟原虫RON 2氨基酸序列为诱饵，筛选间日疟原虫基因组中同源蛋白的编码基因。在恶性疟原虫、间日疟原虫和PlasmoDB和桑格研究所数据库中可获得的其他疟原虫基因组中，确定ron 2及其侧翼基因的基因同线性以及同一性和相似性值。通过从间日疟原虫VCG-1菌株获得的cDNA的RT-PCR确定Pvron 2基因转录。蛋白质表达和定位进行了评估，通过Western印迹和免疫荧光使用多克隆抗PvRON 2抗体。使用针对特异性微线体和棒状体颈蛋白的抗体确认共定位。第一个间日疟原虫棒状体颈蛋白（在此命名为PvRON 2）已在本研究中鉴定。PvRON 2是一种2，204个残基长的蛋白质，由单个6，615 bp的外显子编码，含有朝向氨基末端的疏水性信号序列、朝向羧基末端的跨膜结构域和两个卷曲螺旋α螺旋基序;这些是几种先前描述的针对疟疾的候选疫苗的特征。这种蛋白质在种间可变序列内也含有两个串联重复序列，可能参与逃避宿主的免疫系统。PvRON 2在晚期轴突中表达，并定位于棒状体颈部，类似于已报道的PfRON 2，这表明其参与靶细胞侵袭。本研究描述了第一个间日疟原虫棒状体颈蛋白的鉴定和部分特征。该蛋白质与PfRON 2同源，此前已证明PfRON 2与PfAMA-1相关，这表明PvRON 2也具有类似的作用。
Rhoptries are specialized organelles from parasites belonging to the phylum Apicomplexa; they secrete their protein content during invasion of host target cells and are sorted into discrete subcompartments within rhoptry neck or bulb. This distribution is associated with these proteins' role in tight junction (TJ) and parasitophorous vacuole (PV) formation, respectively. Plasmodium falciparum RON2 amino acid sequence was used as bait for screening the codifying gene for the homologous protein in the Plasmodium vivax genome. Gene synteny, as well as identity and similarity values, were determined for ron2 and its flanking genes among P. falciparum, P. vivax and other malarial parasite genomes available at PlasmoDB and Sanger Institute databases. Pvron2 gene transcription was determined by RT-PCR of cDNA obtained from the P. vivax VCG-1 strain. Protein expression and localization were assessed by Western blot and immunofluorescence using polyclonal anti-PvRON2 antibodies. Co-localization was confirmed using antibodies directed towards specific microneme and rhoptry neck proteins. The first P. vivax rhoptry neck protein (named here PvRON2) has been identified in this study. PvRON2 is a 2,204 residue-long protein encoded by a single 6,615 bp exon containing a hydrophobic signal sequence towards the amino-terminus, a transmembrane domain towards the carboxy-terminus and two coiled coil α-helical motifs; these are characteristic features of several previously described vaccine candidates against malaria. This protein also contains two tandem repeats within the interspecies variable sequence possibly involved in evading a host's immune system. PvRON2 is expressed in late schizonts and localized in rhoptry necks similar to what has been reported for PfRON2, which suggests its participation during target cell invasion. The identification and partial characterization of the first P. vivax rhoptry neck protein are described in the present study. This protein is homologous to PfRON2 which has previously been shown to be associated with PfAMA-1, suggesting a similar role for PvRON2.
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作者：
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