nirK-harboring denitrifiers are more responsive to denitrification- inducing conditions in rice paddy soil than nirS-harboring bacteria.
nirK-harboring denitrifiers are more responsive to denitrification- inducing conditions in rice paddy soil than nirS-harboring bacteria.
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DOI：
10.1264/jsme2.me09160
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发表时间：
2010-03
影响因子：
2.2
通讯作者：
Megumi Yoshida;S. Ishii;S. Otsuka;K. Senoo
中科院分区：
文献类型：
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作者：
Megumi Yoshida;S. Ishii;S. Otsuka;K. Senoo
分析了反硝化诱导前后水稻土亚硝酸盐还原酶基因（nirS和nirK）的数量和多样性。定量PCR分析表明，nirK的拷贝数，但不是nirS，显着增加，在响应的诱导条件。通过克隆库分析鉴定了不同的nirS和nirK克隆。与Burkholderiales和Rhodocyclales的NirS相关的克隆以及与已知反硝化菌远缘的NirK在反硝化诱导条件下增加了它们的比例，因此，可能参与水稻土中的反硝化作用。
We analyzed the quantity and diversity of nitrite reductase genes (nirS and nirK) in rice paddy soil before and after inducing denitrification. A quantitative PCR analysis showed that the copy number of nirK, but not nirS, increased significantly in response to the denitrification-inducing conditions. Diverse nirS and nirK clones were identified by clone library analyses. Clones related to the NirS of Burkholderiales and Rhodocyclales, and NirK distantly related to known denitrifiers increased their proportion in response to the denitrification-inducing conditions, and therefore, might be involved in denitrification in rice paddy soil.